R1256AP Mouse IgG (H+L chain)

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1 mg / €210.00
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R1256AP

Product Description for Mouse IgG

Properties: (H+L chain)
Presentation: AP

Properties for Mouse IgG

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Mouse Immunoglobulin G
Presentation AP
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 �g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Concentration 0.7 mg/ml (by UV absorbance at 280 nm)
Product Citations Purified antibody is cited in:
1. Carvalho JJ, Walter MA, Baermann-Stapel Y, Weller MG, Panne U, Schenk JA, et al. Non-invasive monitoring of immunization progress in mice via IgG from feces. In Vivo. 2012 Jan-Feb;26(1):63-9. PubMed PMID: 22210717.
General Readings Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Liquid (sterile filtered) purified Ig fraction.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Mouse IgG and Mouse Serum.
Targets