Product Description for Mouse IgG
Properties for Mouse IgG
|Product Category||Secondary Antibodies|
|Synonyms||Mouse Immunoglobulin G|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Application||Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 �g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
|Concentration||0.7 mg/ml (by UV absorbance at 280 nm)|
|Product Citations||Purified antibody is cited in:
1. Carvalho JJ, Walter MA, Baermann-Stapel Y, Weller MG, Panne U, Schenk JA, et al. Non-invasive monitoring of immunization progress in mice via IgG from feces. In Vivo. 2012 Jan-Feb;26(1):63-9. PubMed PMID: 22210717.
|General Readings||Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)|
|Storage||Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.