Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed - R1403R | acris-antibodies.com

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Home » Secondary Antibodies » Mouse IgG

Product Description for Mouse IgG

Properties: (H+L chain), F(ab)2 Fragment, adsorbed
Presentation: PE

Properties for Mouse IgG

Product Category	Secondary Antibodies
Quantity	1 ml
Synonyms	Mouse Immunoglobulin G
Presentation	PE
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Mouse IgG whole molecule
Add. information	OD566nm/OD280nm: 2.8
Application	Immunofluorescence: 1/100 - 1/250 Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Concentration	0.5 mg/ml (determined by absorbance = 8.2 at 565 nm for a 0.1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf life: one year from despatch.
Format	Label: R-Phycoerythrin ( (Molecular Weight 240,000 daltons) from seaweed PE Absorption emission: 488 nm / 575 nm Purification: Immunoaffinity Chromatography. Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative. State: Lyophilized purified F(ab')2 Fragment Reconstitution: Restore with 1.0 ml of deionized water (or equivalent)
Specificity	Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Targets	Mouse IgG antibody

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