Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed - R1406R | acris-antibodies.com

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Home » Secondary Antibodies » Mouse IgG

Product Description for Mouse IgG

Properties: (Fc specific), F(ab)2 Fragment, adsorbed
Presentation: PE
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for Mouse IgG

Product Category	Secondary Antibodies
Quantity	1 ml
Synonyms	Mouse Immunoglobulin G
Presentation	PE
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Human IgG F(c) fragment.
Application	Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10⁶ cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications. Recommended Dilutions: Immunofluorescence: 1/100-1/250. Flow Cytometry: 1/100-1/250.
Concentration	0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
General Readings	Carlsson J, Drevin H, Axén R. Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. Biochem J. 1978 Sep 1;173(3):723-37. PubMed PMID: 708370.
Storage	This antibody is stable at 2-8°C as an undiluted liquid. DO NOT FREEZE! Restore with 1.0 ml of deionized water (or equivalent). Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Format	Label: R-Phycoerythrin ( (Molecular Weight 240,000 daltons) PE Absorption emission: 488 nm / 575 nm Purification: Immunoaffinity Chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Preservatives: 0.01% (w/v) Sodium Azide Stabilizers: 10 mg/ml BSA (IgG and Protease free) State: Lyophilized F(ab')2 fragments Reconstitution: Restore with 1.0 ml deionized Water (or equivalent).
Specificity	Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab')2 or Bovine, Horse or Human Serum Proteins. Species: Mouse. Minimum Cross reactivity to Bovine, Horse and Human Serum Proteins.
Targets	Mouse IgG antibody

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