R1361AP Mouse IgG2b (gamma 2b chain specific)

Search for all "Mouse IgG2b"

1 mg / €250.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

R1361AP

Product Description for Mouse IgG2b

Properties: (gamma 2b chain specific)
Presentation: AP

Properties for Mouse IgG2b

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Mouse Immunoglobulin G2b
Presentation AP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG2b heavy chain.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/8,000-1/32,000.
Western Blot: 1/1,000-1/3,000.
Immunohistochemistry: 1/200-1/1,000.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings (Conjugation) Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
Storage Store the antibody (undiluted) at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial
loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative.
State:
Liquid (sterile filtered) purified IgG fraction.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum.
Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Targets