R1253F Mouse IgG (H+L chain) antibody

Search for all "Mouse IgG"

2 mg / €190.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

R1253F

Product Description for Mouse IgG

Properties: (H+L chain)
Presentation: FITC
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay.

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Mouse Immunoglobulin G
Presentation FITC
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule.
Application Suitable for ELISA, Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
Flow Cytometry: 1/2,000-1/10,000.
IF Microscopy: 1/500-1/2,500.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate (Molecular Weight 390 daltons).Conjugation Chemistry: Isothiocyanate
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
3.1 moles FITC per mole of Rabbit IgG
Purification:
Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (Ig and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified IgG fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Species:
Mouse.
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