R1302AP Chicken IgG / Chicken IgY (F(ab)2 specific)

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1 mg / €240.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

R1302AP

Product Description for Chicken IgG / Chicken IgY

Properties: (F(ab)2 specific)
Presentation: AP

Properties for Chicken IgG / Chicken IgY

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Chicken Immunoglobulin G, Chicken Immunoglobulin Y
Presentation AP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Chicken IgG F(ab')2 fragment.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.
Concentration 0.6 mg/ml (by UV absorbance at 280 nm)
General Readings Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation).
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine, Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.05 M Tris Chloride, 0.15 M Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) and 0.01% (w/v) and 0.01% Sodium Azide as preservative.
Purity:
/
State:
Liquid (sterile filtered) purified Ig fraction.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum.
No reaction was observed against Chicken IgG F(c).
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