R1427R Rabbit IgG F(ab)2 Fragment (H+L chain)

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0.5 mg / €320.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

R1427R

Product Description for Rabbit IgG

Properties: F(ab)2 Fragment (H+L chain)
Presentation: PE
Product is tested for Flow Cytometry.

Properties for Rabbit IgG

Product Category Secondary Antibodies
Target Category
Quantity 0.5 mg
Synonyms Rabbit Immunoglobulin G
Presentation PE
Clonality Polyclonal
Host Donkey
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rabbit IgG whole molecule.
Application Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Concentration 0.5 mg/ml (determined by absorbance = 82.0 at 565
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R-Phycoerythrin ( Molecular Weight 240,000 daltons)
PE
Absorption emission:
488 nm / 575 nm
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride; pH 7.2 containing 10 mg/ml BSA (Immunoglobulin and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
State:
Lyophilized F(ab')2 fragments.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.
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