Rabbit IgA+IgG+IgM (H+L chain) - AP21490SU-N | acris-antibodies.com

JavaScript seems to be disabled in your browser.
You must have JavaScript enabled in your browser to utilize the functionality of this website.

Home » Secondary Antibodies » Rabbit IgA+IgG+IgM

Product Description for Rabbit IgA+IgG+IgM

Properties: (H+L chain)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Rabbit IgA+IgG+IgM

Product Category	Secondary Antibodies
Quantity	1 ml
Synonyms	Rabbit IgA, Rabbit IgG, Rabbit IgM
Presentation	Serum
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Purified normal IgG and fractions containing IgA, and IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application	Can be used in precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal rabbit serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Recommended dilutions: Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum. Double radial immunodiffusion: a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal rabbit serum in agar block titration.
Concentration	Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Storage	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	State: Lyophilized (Delipidated and heat inactivated) stable whole serum - No preservative added Serum Reconstitution: Restore by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding PBS, pH 7.2
Specificity	Specificity: The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Service

Acris Antibodies GmbH
Schillerstraße 5
D-32052 Herford
Germany
phone: +49-5221-34606-0
free call: 0800-227 47 46 (Germany only)
fax: +49-5221-34606-11
email: info@acris-online.de
orders: ordering@acris-online.de

© Copyright 1998 - 2013 Acris Antibodies GmbH