Rabbit IgG (H+L chain) - R1364F | acris-antibodies.com

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Home » Secondary Antibodies » Rabbit IgG

Product Description for Rabbit IgG

Properties: (H+L chain)
Presentation: FITC
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for Rabbit IgG

Product Category	Secondary Antibodies
Quantity	2 mg
Synonyms	Rabbit Immunoglobulin G
Presentation	FITC
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Rabbit IgG, whole molecule.
Application	This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Immunofluoresence: 1/1,000-1/5,000.
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Product Citations	HRP conjugated antibody is cited in: 1. Kokk K, Kuuslahti M, Keisala T, Purmonen S, Kaipia A, Tammela T, et al. Expression of luteinizing hormone receptors in the mouse penis. J Androl. 2011 Jan-Feb;32(1):49-54. doi: 10.2164/jandrol.109.008623. Epub 2010 Aug 26. PubMed PMID: 20798391.
General Readings	The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage	Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after restoration and standing at RT. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Protect from light. Shelf life: One year from despatch.
Format	Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) FITC Absorption emission: 495 nm / 528 nm Molar radio: 3.7 moles FITC per mole of Goat IgG Purification: Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Preservatives: 0.01% (w/v) Sodium Azide Stabilizers: 10 mg/ml BSA (IgG and Protease free) State: Lyophilized purified Ig fraction Reconstitution: Restore with 1.0 ml of deionized water or equivalent.
Specificity	Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Targets	Rabbit IgG antibody

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