R1364HRP Rabbit IgG (H+L chain)

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2 mg / €180.00
Delivery Time: 24 hours

Product Description for Rabbit IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Rabbit IgG

Product Category Secondary Antibodies
Quantity 2 mg
Synonyms Rabbit Immunoglobulin G
Presentation HRP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Rabbit IgG, whole molecule
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/200,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Caution Do Not Add Sodium Azide.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
General Readings
  • Farr & Nakane, J. Immunol. Methods 47; 129-144, 1981. (Conjugation)
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin Sulfate
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Targets