Rat IgG (H+L chain) - R1371AP | acris-antibodies.com

Product Description for Rat IgG

Properties: (H+L chain)
Presentation: AP

Immunogen	Immunogen: Rat IgG whole molecule.
Application	Suitable for Immunoblotting (Dot blot or Western blot, 1/500-1/2,500), ELISA (1/2,000-1/10,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Concentration	1.0 mg/ml (by UV absorbance at 280 nm)
General Readings	Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971
Storage	Store the antibody (undiluted) at 2-8°C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: one year from despatch.
Format	Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) AP Purification: Immunoaffinity chromatography. Buffer System: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 containing 0.01% (w/v) Sodium Azide as preservative. State: Liquid (sterile filtered) purified Ig fraction.
Specificity	Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG and Rat Serum.
Targets	Rat IgG antibody