R1372AP Rat IgG (H+L chain), adsorbed

Search for all "Rat IgG"

0.5 mg / €220.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

R1372AP

Product Description for Rat IgG

Properties: (H+L chain), adsorbed
Presentation: AP

Properties for Rat IgG

Product Category Secondary Antibodies
Quantity 0.5 mg
Synonyms Rat Immunoglobulin G
Presentation AP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Rat IgG whole molecule.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:7,000 of the reconstitution concentration is suggested for this product.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  • Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
Storage Store the antibody (undiluted) at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial
loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer, IgG and Protease free.
State:
Liquid (sterile filtered) purified Ig fraction
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG and Rat Serum.
No reaction was observed against Human Serum Proteins.
Targets