Rat IgG F(ab)2 specific (adsorbed F(ab)2 Fragment) - R1415HRP | acris-antibodies.com

JavaScript seems to be disabled in your browser.
You must have JavaScript enabled in your browser to utilize the functionality of this website.

Home » Secondary Antibodies » Rat IgG

Product Description for Rat IgG

Properties: F(ab)2 specific (adsorbed F(ab)2 Fragment)
Presentation: HRP

Properties for Rat IgG

Product Category	Secondary Antibodies
Quantity	0.5 ml
Synonyms	Rat Immunoglobulin G
Presentation	HRP
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Rat IgG F(ab')2 fragment
Application	Suitable for Immunoblotting (Western, 1:1,000-1:10,000 or dot blot), ELISA (1:10,000-1:100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:5,000). as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Concentration	1.0 mg/ml (by UV absorbance at 280 nm)
General Readings	Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Storage	Store vial at 2-8°C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Format	Label: Horseradish Peroxidase HRP Purification: Immunoaffinity chromatography. Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. State: Lyophilized F(ab')2 fragments. Reconstitution: Restore with 0.5 ml of deionized water (or equivalent).
Specificity	Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Targets	Rat IgG antibody

Service

Acris Antibodies GmbH
Schillerstraße 5
D-32052 Herford
Germany
phone: +49-5221-34606-0
free call: 0800-227 47 46 (Germany only)
fax: +49-5221-34606-11
email: info@acris-online.de
orders: ordering@acris-online.de

© Copyright 1998 - 2013 Acris Antibodies GmbH