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Product Description for Fibroplasts/Epithelial Cells
Properties for Fibroplasts/Epithelial Cells
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.2 mg |
| Reactivity | Human |
| Presentation | Purified |
| Applications | Frozen sections, Flow cytometry, Immunoprecipitation |
| Isotype | IgG2a |
| Host | Mouse |
| Clone | D7-FIB |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Fibroblast Marker, Fibroblasten, FocusOn008 |
| Immunogen | Human foreskin fibroblasts |
| Product Type | Antibodies |
| Recommended Isotype Control | AM03096PU-N |
| Storage | Store at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Fearns, C. and Dowdle, E. B. (1992). The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro. Int. J. Cancer. 50:621-627. 2. Kelynack, K. J. et al. (2000). Human renal fibroblast contraction of collage I lattices is an integrin-mediated process. Nephrol. Dial. Transplant. 15:1766-1772. 3. Van Osch, G. J. V. M. , et al. (2001). Monoclonal antibody 11-Fibrau: A useful marker to characterize chondrocyte differentiation stage. Biochem. Biophys. Res Commun. 280:806-812. |
| Format | State: Liquid purified IgG |
| Purification: Protein G chromatography | |
| Buffer System: PBS, pH 7.4, containing 0.09% sodium azide as preservative | |
| Specificity | SM1214P recognises a 112 kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS but resistant to trypsin, tunicamycin and collagenase. In immunohistochemical studies the antibody has also been found to bind to epithelium, myoepithelium, smooth muscle cells and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). |
| Application | Flow cytometry: use 10 µl of a 1:50 - 1:200 dilution to label 10e6 cells in 100 µl. This product is routinely tested in flow cytometry on the KG1 cell line. Immunohistochemistry on frozen tissues or cells (1:100): use ice-cold methanol for 5 min as fixative. Does not work on paraffin sections. Can also be used for immunoprecipitation. |
| Concentration | 1.0 mg/ml |
| Focuson | 008 Fibroblast Marker Antibodies - FocusOn No.8 |
Secondary Antibodies for Fibroplasts/Epithelial Cells
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 190 Euro | |||||
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170 Euro | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160 Euro | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 180 Euro | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170 Euro | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Rabbit | PE | 1 ml / 290 Euro | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Goat | PE | 1 ml / 290 Euro | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 270 Euro | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
