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Product Description for CD1a
T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen
Properties for CD1a
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.1 mg |
| Reactivity | Human |
| Presentation | Purified |
| Applications | Frozen sections, Flow cytometry |
| Isotype | IgG1 |
| Host | Mouse |
| Clone | HI149 |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | T6/Leu-6, hTa1, FocusOn109 |
| Swiss Prot Num | P06126 |
| Immunogen | Human thymocytes |
| Product Type | Antibodies |
| Recommended Isotype Control | SM10P (for use in human samples), AM03095PU-N |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Sugita M, Grant EP, van Donselaar E, Hsu VW, Rogers RA, Peters PJ, Brenner MB.: Separate pathways for antigen presentation by CD1 molecules. Immunity. 1999 Dec;11(6):743-52. 2. Hiromatsu K, Dascher CC, Sugita M, Gingrich-Baker C, Behar SM, LeClair KP, Brenner MB, Porcelli SA.: Characterization of guinea-pig group 1 CD1 proteins. Immunology. 2002 Jun;106(2):159-72. 3. Raftery MJ, Winau F, Kaufmann SH, Schaible UE, Schönrich G.: CD1 antigen presentation by human dendritic cells as a target for herpes simplex virus immune evasion. J Immunol. 2006 Nov 1;177(9):6207-14. 4. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). 5. Favali C, Tavares N, Clarêncio J, Barral A, Barral-Netto M, Brodskyn C: Leishmania amazonensis infection impairs differentiation and function of human dendritic cells. J Leukoc Biol. 2007 Dec;82(6):1401-6. 6. Hunger RE, Sieling PA, Ochoa MT, Sugaya M, Burdick AE, Rea TH, Brennan PJ, Belisle JT, Blauvelt A, Porcelli SA, Modlin RL: Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells. J Clin Invest. 2004 Mar;113(5):701-8. 7. Mayer WJ, Irschick UM, Moser P, Wurm M, Huemer HP, Romani N, Irschick EU: Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers. Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4459-67. 8. Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol. 2007 Nov;19(11):1271-9. 9. Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol. 2005 Mar;32(3):177-84. 10. Chen X, Murakami T, Oppenheim JJ, Howard OM: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking. Blood. 2005 Oct 1;106(7):2409-16. 11. McKenna RW, Asplund SL, Kroft SH: Immunophenotypic analysis of hematogones (B-lymphocyte precursors) and neoplastic lymphoblasts by 4-color flow cytometry. Leuk Lymphoma. 2004 Feb;45(2):277-85. 12. Perros F, Dorfmüller P, Souza R, Durand-Gasselin I, Mussot S, Mazmanian M, Hervé P, Emilie D, Simonneau G, Humbert M: Dendritic cell recruitment in lesions of human and experimental pulmonary hypertension. Eur Respir J. 2007 Mar;29(3):462-8. 13. Säemann MD, Parolini O, Böhmig GA, Kelemen P, Krieger PM, Neumüller J, Knarr K, Kammlander W, Hörl WH, Diakos C, Stuhlmeier K, Zlabinger GJ: Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells. J Leukoc Biol. 2002 Feb;71(2):238-46. 14. Kirsch BM, Zeyda M, Stuhlmeier K, Grisar J, Smolen JS, Watschinger B, Stulnig TM, Hörl WH, Zlabinger GJ, Säemann MD: The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function. Arthritis Res Ther. 2005;7(3):R694-703. |
| Format | State: Liquid Ig fraction |
| Purification: Protein G-affinity chromatography; purity: > 95% (by SDS-PAGE) | |
| Buffer System: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 | |
| Specificity | The antibody reacts with CD1a (T6), a 49 KDa polypeptide associated with b2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes. |
| Application | Flow cytometry. Immunohistochemistry on frozen sections. |
| Background | CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification. |
| Concentration | 1.0 mg/ml |
| Focuson | 109 Antibodies for Characterization of Dendritic Cells |
Secondary Antibodies for CD1a
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 190 Euro | |||||
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170 Euro | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160 Euro | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 180 Euro | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170 Euro | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Rabbit | PE | 1 ml / 290 Euro | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Goat | PE | 1 ml / 290 Euro | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 270 Euro | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
