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SM6021: <b>Western blot analysis</b>: Human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL4 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system.  <b>Lane 1 :</b> Extracellular domain of KIR2DL1(23-223aa) <b>Lane 2 :</b> Extracellular domain of KIR2DL3(23-223aa) <b>Lane 3 :</b> Extracellular domain of KIR2DL4(44-202aa)

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SM6021 CD158d / KIR2DL4 (L1, L3, L4, S4) antibody

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0.1 ml / €310.00
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Delivery time: 5-7 workingdays
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Quick Overview

Mouse anti Human CD158d / KIR2DL4 2H6

Product Description for CD158d / KIR2DL4


Killer cell immunoglobulin-like receptor 2DL4, MHC class I NK cell receptor KIR103AS, Killer cell inhibitory receptor 103AS, CD158 antigen-like family member D

Properties for CD158d / KIR2DL4

ManufacturerAcris Antibodies GmbH
Quantity0.1 ml
ReactivityHuman
PresentationPurified
ApplicationsEnzyme Immunoassay, Western blot / Immunoblot
IsotypeIgG2b
HostMouse
Clone2H6
Shipping toWorldwide

Datasheet Extract

SynonymsKIR103AS, G9P, FocusOn037, FocusOn114
Swiss Prot Num Q99706
Immunogen Recombinant human KIR2DL4 purified from E. coli
Product TypeAntibodies
Recommended Isotype ControlSM12P, AM03110PU-N
StorageStore the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Shin JS, Shin EC, Kim J, Choi IH, Park JH, Kim SJ. 1999. Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors. Hybridoma 18:521-527.
2. Chwae YJ, Chang MJ, Park SM, Yoon H, Park HJ, Kim SJ, Kim J. 2002 Molecular mechanism of the activation-induced cell death inhibition mediated by a p70 inhibitory killer cell Ig-like receptor in Jurkat T cells. J Immunology 169:3726-3735.
FormatState: Liquid purified Ig fraction
 Purification: Protein A affinity chromatography
 Buffer System: PBS, pH 7.4, containing 0.09 % sodium azide
Specificity This antibody recognizes KIR2DL1, KIR2DL3, KIR2DL4 and KIR2DS4, members of killer cell immunoglobulin-like receptor family: ELISA KIR2DL1: ++ KIR2DL3: + KIR2DL4: +++ KIR2DS4: ++ Western blot (1:500 - 1:2,000) KIR2DL1: +++ KIR2DL3: +++ KIR2DL4: ++ KIR2DS4: ++
ApplicationELISA.
Western blot.
BackgroundThe KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.
Concentration1.0 mg/ml
Focuson 037 Antibodies to NK-cells: CD335 (NKp46), CD305 (LAIR-1) and KIR – Family
Focuson 114 NK-Cell Markers

Secondary Antibodies for CD158d / KIR2DL4

Catalog No.Name / HostPresentationReactivity 
R1253AP Mouse IgG (H&L)
  Rabbit AP 1 mg / 190 Euro
   
R1253B Mouse IgG (H&L)
  Rabbit Biotin 2 mg / 170 Euro
   
R1253F Mouse IgG (H&L)
  Rabbit FITC 2 mg / 160 Euro
   
R1253HRP Mouse IgG (H&L)
  Rabbit HRP 2 mg / 180 Euro
   
R1253TR Mouse IgG (H&L)
  Rabbit Texas Red 2 mg / 170 Euro
   
R1403R Mouse IgG (H&L) F(ab&#039;)2 Fragment
  Rabbit PE 1 ml / 290 Euro
   
R1405R Mouse IgG (H&L) F(ab&#039;)2 Fragment
  Goat PE 1 ml / 290 Euro
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.
  Donkey PE 1 ml / 290 Euro
   
R1457C2 Mouse IgG (H&L) multi-species ads.
  Goat Cy2 1 mg / 270 Euro
   
R1457C3 Mouse IgG (H&L) multi-species ads.
  Goat Cy3 1 mg / 250 Euro
   
R1457C5 Mouse IgG (H&L) multi-species ads.
  Goat Cy5 1 mg / 250 Euro
   
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