6xHistidine Epitope Tag
Principal name
6xHistidine Epitope Tag antibody
Selected pictures


Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))
Background of 6xHistidine Epitope Tag antibody
In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.
General readings
1. Conrath, K. et al. (2001) Camel single binding-domain antibodies as modular binding units in bispecific and bivalent antibody constructs. J. Biol.Chem. 276 (10): 7346-7350
2. Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice. Immunol. 103: 301-309.
3. Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells. J. Biol. Chem. 282: 22370-22375.
4. Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen. BMC Immunol. 8: 4.
5. Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization. Genes Brain Behav. 7: 244-256.
6. Wrighton, K.H. et al. (2009) Transforming Growth Factor beta can stimulate Smad1 phosphorylation independently of Bone Morphogenic Protein receptors. J. Biol. Chem. 284: 9755-9763.
7. Diefenbacher, M. et al., The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae. J Biol Chem. 286: 25027-25038 (2011).
8. Alvarez, M.M. et al., Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. PLoS One. 5: e10176 (2010).
9. Bahi, A. et al., The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement. Neuropsychopharmacology 33: 2726-2735 (2008).
10. Gunnarsen, K.S. et al., Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnol. 10: 8 (2010).
11. Hwang, H.Y. et al., Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment. Mol Immunol. 45: 2570-2580 (2008).
12. De Vooght, L. et al., Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly. Microb Cell Fact. 11: 23 (2012).
Antibodies
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| AM00195PU-N |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | BV-G020 | ICC/IF, IP, WB |
0.1 mg /
€370.00
|
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| IgG2b | Acris Antibodies GmbH | ||||||
+1 additional image | |||||||
| AM05288PU-N |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | IPA2C5.1 | Purified | WB |
0.1 mg /
€300.00
|
||
| IgG2a | Acris Antibodies GmbH | ||||||
| AM11011SU-N |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | 6AT18 | Ascites | E, WB |
0.1 mg /
€330.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| DP3514 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Rabbit | Aff - Purified | WB |
50 µg /
€180.00
|
|||
| Acris Antibodies GmbH | |||||||
+1 additional image | |||||||
| DP3524 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Rabbit | Purified | WB |
50 µg /
€180.00
|
|||
| Acris Antibodies GmbH | |||||||
| R1181 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Rabbit | Aff - Purified | C, E, WB |
0.1 mg /
€240.00
|
|||
| Acris Antibodies GmbH | |||||||
| R1181B |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Rabbit | Biotin | C, E, WB |
0.1 mg /
€320.00
|
|||
| Acris Antibodies GmbH | |||||||
| R1181HRP |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Rabbit | HRP | C, E, WB |
0.1 mg /
€320.00
|
|||
| Acris Antibodies GmbH | |||||||
| SM1693B |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | Biotin | C, E, IP, WB |
0.1 mg /
€520.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| SM1693F |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | FITC | F, ICC/IF |
0.1 mg /
€390.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| SM1693HRP |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | HRP | E, WB |
0.1 mg /
€520.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| SM1693P |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | Purified | C, E, IP, WB |
1 mg /
€530.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| SM1693PS |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | Purified | C, E, IP, WB |
0.1 mg /
€250.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| SM1693PX |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | Purified | C, E, IP, WB |
2 mg /
€1,120.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| NB100-63172 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | Biotin | E, WB |
0.1 mg /
€330.00
|
||
| IgG1 | Novus Biologicals Inc. | ||||||
| NB100-63173 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
![]() |
Mouse | AD1.1.10 | HRP | E, WB |
0.1 mg /
€330.00
|
||
| IgG1 | Novus Biologicals Inc. | ||||||
+2 additional images | |||||||
| NB100-64150 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | FITC | F |
Regular Price: 0.1 mg / €330.00 Special Price: 0.1 mg / €270.00 |
||
| IgG1 | Novus Biologicals Inc. | ||||||
| NB100-64151 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | PE | F |
Regular Price: 0.1 ml / €460.00 Special Price: 0.1 ml / €370.00 |
||
| IgG1 | Novus Biologicals Inc. | ||||||
| NB100-64768 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | AD1.1.10 | Purified | All Species | C, E, IP |
Regular Price: 0.1 mg / €330.00 Special Price: 0.1 mg / €270.00 |
|
| IgG1 | Novus Biologicals Inc. | ||||||
| NB100-72978 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
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Mouse | His17 | Purified | All Species | E, IP, WB |
0.2 mg /
€230.00
|
|
| IgG1 | Novus Biologicals Inc. | ||||||


+1 additional image




![Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/0164033.jpeg)
![Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/0164052.jpeg)





![Western Blot: HISTIDINE TAG Antibody (AD1.1.10) [NB100-63172] - Western blot of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/p_63172_1.jpg)
![Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - Western blot of HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/6-Histidine-Epitope-Tag-Antibody-(AD1.1.10)-[HRP]-Western-Blot-NB100-63173-img0004.jpg)

![Flow Cytometry: 6-Histidine Epitope Tag Antibody (AD1.1.10) [PE] [NB100-64151] - Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/6-Histidine-Epitope-Tag-Antibody-(AD1.1.10)-[PE]-Flow-Cytometry-NB100-64151-img0001.jpg)
![Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [NB100-64768] - Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [NB100-64768] - Western blot analysis of 1) Histidine tagged FLCN protein and 2) Histidine tagged homer protein.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/6-Histidine-Epitope-Tag-Antibody-(AD1.1.10)-Western-Blot-NB100-64768-img0001.jpg)