6xHistidine Epitope Tag
Principal name
6xHistidine Epitope Tag antibody
Selected pictures
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))
Background of 6xHistidine Epitope Tag antibody
In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.
General readings
1. Conrath, K. et al. (2001) Camel single binding-domain antibodies as modular binding units in bispecific and bivalent antibody constructs.
J. Biol.Chem. 276 (10): 7346 - 7350
2. Bahi, A. et al. (2004) CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus.
Eur. J. Neurosci. 19:1621-1633.
Antibodies
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| NB100-64768 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
![Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [NB100-64768] - Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [NB100-64768] - Western blot analysis of 1) Histidine tagged FLCN protein and 2) Histidine tagged homer protein.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/6-Histidine-Epitope-Tag-Antibody-(AD1.1.10)-Western-Blot-NB100-64768-img0001.jpg) |
Mouse | AD1.1.10 | Purified | All Species | C, E, IP |
Regular Price: 0.1 mg / €330.00 Special Price: 0.1 mg / €270.00 |
|
| IgG1 | Novus Biologicals Inc. | ||||||
| NB100-72978 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
 |
Mouse | His17 | Purified | All Species | E, IP, WB |
0.2 mg /
€230.00
|
|
| IgG1 | Novus Biologicals Inc. | ||||||
| NBP1-25939 |
6xHistidine Epitope Tag (HHHHHH) antibody | ||||||
|
Goat | Aff - Purified | All Species | E, ICC/IF, IP, WB |
0.1 mg /
€280.00
|
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| IgG | Novus Biologicals Inc. | ||||||
