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6xHistidine Epitope Tag

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Principal name

6xHistidine Epitope Tag antibody

Selected pictures

6xHistidine Epitope Tag antibody - AM00195PU-N
AM00195PU-N - Western blot analysis of His in 293 cells transfectedwith His-tagged vector (Lane 2) and Control vector (Lane 1).
6xHistidine Epitope Tag antibody - AM00195PU-N
AM00195PU-N - Immunofluorescence of His-tagged fusion protein expressed in 293 cells. Left Lane: Untransfected control; Right Lane: Transfected cells.

Available reactivities

All Species, Hu (Human)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of 6xHistidine Epitope Tag antibody

In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag” (His-tag) is the most used affinity epitope for the purification of recombinant proteins [1]. Proteins with a polyhistidine tag (e.g. 6xHis or 8xHis) can be purified in one step using a metal-chelate column (e.g. Ni2+, Zn2+, Cu2+ or Co2+) and imidazole as eluent. This method now is a very attractive system for the purification of larger amounts proteins for structural and functional studies. So far His-tagged proteins were successfully purified from different expression systems like E. coli, yeast, insect cells and plant cells [1,2]. An important requirement beside the efficient and robust purification method is the availability of a fast detection system for checking the purification steps of these His-tagged proteins if no specific antibody is available.

General readings

1. Conrath, K. et al. (2001) Camel single binding-domain antibodies as modular binding units in bispecific and bivalent antibody constructs.
J. Biol.Chem. 276 (10): 7346 - 7350
2. Bahi, A. et al. (2004) CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus.
Eur. J. Neurosci. 19:1621-1633.

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AM00195PU-N

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot analysis of His in 293 cells transfectedwith His-tagged vector (Lane 2) and Control vector (Lane 1). Mouse BV-G020 ICC/IF, IP, WB
0.1 mg / €370.00
  IgG2b   Acris Antibodies GmbH
+1 additional image
 
AM05288PU-N

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. Western blot of His tagged protein (vector pET 30C) using AM05288PU-N antibody. Lane 1: Whole cellular extract of BL21 (DE3) pJT4pTT9 bacteria carrying gene encoding for His-Rel E protein (uninduced). Lane 2: Induced. Lane 3: Induced (1/2 dilution). Lane 4: Purified His-Rel E protein (~ 17 kDa). Mouse IPA2C5.1 Purified WB
0.1 mg / €300.00
  IgG2a   Acris Antibodies GmbH
 
AM11011SU-N

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS monoclonal antibody (6AT18). Mouse 6AT18 Ascites E, WB
0.1 mg / €330.00
  IgG1   Acris Antibodies GmbH
 
DP3514

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot (ECL) detection of different His-tagged proteins. For Western blot analysis each lane was loaded with 12,5-50 ng with the following His-tagged proteins: Lane 1: TbTX, Lane 2: IL-3, Lane 3: VEGF121, Lane 4: PlGF-1, Lane 5: Lac I repressor, Lane 6: soluble FLT-4. Rabbit Aff - Purified WB
50 µg / €180.00
    Acris Antibodies GmbH
+1 additional image
 
DP3524

6xHistidine Epitope Tag (HHHHHH) antibody

6xHistidine Epitope Tag Rabbit Purified WB
50 µg / €180.00
    Acris Antibodies GmbH
 
R1181

6xHistidine Epitope Tag (HHHHHH) antibody

Figure 1. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. The blot was blocked with 3% BSA in TBST for 45 min at RT. Antibody was incubated with blot at a 1/1,000 dilution in TBST with 3% BSA for 1 hour at RT. Detection occurred using HRP Goat-anti-Rabbit IgG diluted 1/80,000 in blocking buffer for 30 min at RT. Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~42 kDa). Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~40 kDa). A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. Proteins were transferred to nitrocellulose using standard methods. Molecular weights were estimated by comparison to standards (lane M). Rabbit Aff - Purified C, E, WB
0.1 mg / €240.00
    Acris Antibodies GmbH
 
R1181B

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Biotin C, E, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
R1181HRP

6xHistidine Epitope Tag (HHHHHH) antibody

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit HRP C, E, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
SM1693B

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (SM1693B) and visualised with Streptavidin - HRP Mouse AD1.1.10 Biotin C, E, IP, WB
0.1 mg / €520.00
  IgG1   Acris Antibodies GmbH
 
SM1693F

6xHistidine Epitope Tag (HHHHHH) antibody

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody (SM1693F). Mouse AD1.1.10 FITC F, ICC/IF
0.1 mg / €390.00
  IgG1   Acris Antibodies GmbH
 
SM1693HRP

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HISTIDINE TAG:HRP (SM1693HRP). Mouse AD1.1.10 HRP E, WB
0.1 mg / €520.00
  IgG1   Acris Antibodies GmbH
 
SM1693P

6xHistidine Epitope Tag (HHHHHH) antibody

His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag antibody Cat.-No SM1693P Mouse AD1.1.10 Purified C, E, IP, WB
1 mg / €530.00
  IgG1   Acris Antibodies GmbH
 
SM1693PS

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (SM1693P/PX) visualised with Rabbit anti Mouse IgG -HRP conjugated (SP1012HRP). Mouse AD1.1.10 Purified C, E, IP, WB
0.1 mg / €250.00
  IgG1   Acris Antibodies GmbH
 
SM1693PX

6xHistidine Epitope Tag (HHHHHH) antibody

Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (SM1693P/PX) visualised with Rabbit anti Mouse IgG -HRP conjugated (SP1012HRP). Mouse AD1.1.10 Purified C, E, IP, WB
2 mg / €1,120.00
  IgG1   Acris Antibodies GmbH
 

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