c-Myc Epitope Tag (EQKLISEEDL)

Principal name

c-Myc Epitope Tag (EQKLISEEDL) antibody

Selected pictures

c-Myc Epitope Tag (EQKLISEEDL) antibody - SP5385P
SP5385P - Immunolocalization of c-Myc in transfected mammalian cells using SP5385P
c-Myc Epitope Tag (EQKLISEEDL) antibody - NB600-302 c-Myc Epitope Tag (EQKLISEEDL)
NB600-302 c-Myc Epitope Tag (EQKLISEEDL) - Immunocytochemistry: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

Gene ID

4609 (MYC)

Available reactivities

Dros (Drosophila), Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse, Chicken, Goat, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)

Background of c-Myc Epitope Tag (EQKLISEEDL) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. We offer anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Please visit our website at www.acris-antibodies.de for more informations.

General readings

General / background references:Spandidos DA et al. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res 7:1299-304 (1987). PubMed PMID: 3327455
Evan GI et al.

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Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
R1182

c-Myc Epitope Tag (EQKLISEEDL) antibody

Figure 1. Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by Western blot. Polyclonal rabbit-anti-Myc epitope tag antibody was diluted to 1.0 µg/ml to detect either recombinant protein. 4-20% gradient gels were used to separate the proteins by SDS-PAGE. The proteins were transferred to nitrocellulose using standard methods. After blocking the membranes were probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (Green 800 nm channel). Pre-stained molecular weight markers are also shown (lane M, Red 700 nm channel). LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. 
Rabbit Aff - Purified C, E, WB
0.1 mg / €270.00
    Acris Antibodies GmbH
R1182B

c-Myc Epitope Tag (EQKLISEEDL) antibody

Anti-Myc epitope tag polyclonal antibody detects Myc-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-Myc epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 ug of recombinant protein containing the Myc epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Biotin C, E, WB
0.1 mg / €340.00
    Acris Antibodies GmbH
R1182HRP

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Rabbit HRP C, E, WB
0.1 mg / €340.00
    Acris Antibodies GmbH
SM1535P

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Rat IgG JAC6 Purified C, ICC/IF, IP, WB
0.1 mg / €500.00
    Acris Antibodies GmbH
SM1863AP

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc tagged protein detected with Mouse anti c-Myc antibody AP conjugated (Cat.-No SM1863AP). Mouse IgG1 9E10 AP Hu C, E, P, WB
0.1 mg / €450.00
    Acris Antibodies GmbH
SM1863B

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse IgG1 9E10 Biotin C, E, F, P, WB
0.1 mg / €440.00
    Acris Antibodies GmbH
SM1863F

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse IgG1 9E10 FITC Hu C, F, ICC/IF
0.1 mg / €440.00
    Acris Antibodies GmbH
SM1863HRP

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse IgG1 9E10 HRP Hu C, E, P, WB
0.1 mg / €450.00
    Acris Antibodies GmbH
SM1863P

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse IgG1 9E10 Purified Hu C, E, F, IP, P, WB
1 mg / €600.00
    Acris Antibodies GmbH
SM1863PSX

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse IgG1 9E10 Purified C, E, F, IP, P, WB
0.1 mg / €180.00
    Acris Antibodies GmbH
SM1863PX

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse IgG1 9E10 Purified Hu C, E, F, P, WB
2 mg / €1,240.00
    Acris Antibodies GmbH
SM1863R

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse IgG1 9E10 PE Hu F
100 Tests / €530.00
    Acris Antibodies GmbH
SP5385CP

c-Myc Epitope Tag (EQKLISEEDL) control peptide

c-Myc Epitope Tag (EQKLISEEDL) Purified
50 µg / €180.00
    Acris Antibodies GmbH
SP5385P

c-Myc Epitope Tag (EQKLISEEDL) antibody

Immunolocalization of c-Myc in transfected mammalian cells using SP5385P Rabbit Aff - Purified ICC/IF
0.1 ml / €260.00
    Acris Antibodies GmbH
NB110-58364

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Chicken FITC ICC/IF
0.5 ml / €330.00
    Novus Biologicals Inc.
NB110-58366

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Chicken HRP WB
0.5 ml / €330.00
    Novus Biologicals Inc.
NB600-302

c-Myc Epitope Tag (EQKLISEEDL) antibody

Immunocytochemistry: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody.  Nuclei are counterstained with Hoechst 33258 (Blue). Mouse IgG1 9E10 Purified Dros, Hu, Ms C, E, F, ICC/IF, IP, P, WB
0.1 ml / €340.00
    Novus Biologicals Inc.
NB600-302B

c-Myc Epitope Tag (EQKLISEEDL) antibody

Western Blot: c-Myc Antibody (9E10) [NB600-302] - Western blot analysis of c-myc in Jurkat cell lysates using NB600-302. Mouse IgG1 9E10 Dros, Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / €410.00
    Novus Biologicals Inc.
NB600-302C

c-Myc Epitope Tag (EQKLISEEDL) antibody

Western Blot: c-Myc Antibody (9E10) [NB600-302] - Western blot analysis of c-myc in Jurkat cell lysates using NB600-302. Mouse IgG1 9E10 Dros, Hu, Ms C, E, F, ICC/IF, IP, P, WB
0.1 ml / €410.00
    Novus Biologicals Inc.
NB600-302G

c-Myc Epitope Tag (EQKLISEEDL) antibody

Western Blot: c-Myc Antibody (9E10) [NB600-302] - Western blot analysis of c-myc in Jurkat cell lysates using NB600-302. Mouse IgG1 9E10 Dros, Hu, Ms C, E, F, ICC/IF, IP, P, WB
0.1 ml / €410.00
    Novus Biologicals Inc.

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