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c-Myc Epitope Tag (EQKLISEEDL)

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Principal name

c-Myc Epitope Tag (EQKLISEEDL) antibody

Selected pictures

c-Myc Epitope Tag (EQKLISEEDL) antibody - SP5385P
SP5385P - Immunolocalization of c-Myc in transfected mammalian cells using SP5385P
c-Myc Epitope Tag (EQKLISEEDL) antibody - NB600-302 c-Myc Epitope Tag (EQKLISEEDL)
NB600-302 c-Myc Epitope Tag (EQKLISEEDL) - Immunocytochemistry: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

Gene ID

4609 (MYC)

Ncbi ID

NP_002458, NP_002458.2, 9606

Available reactivities

Dros (Drosophila), Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse, Chicken, Goat, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Functional assay (FN), Immunoelectron Microscopy (EM)

Background of c-Myc Epitope Tag (EQKLISEEDL) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. We offer anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Please visit our website at www.acris-antibodies.de for more informations.

General readings

1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. proto-oncogene product. Mol. Cell. Biol. 5: 3610 -3616.
2. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 7: 1299 -1304.
3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae. Microb. Cell Fact. 9: 74-86.
4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction. Biochem J. 404: 23-9.
5. Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J Cell Biol. 187: 959-66.
6. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 14: 803-6.
7. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins. 73: 552-65.
8. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling. J Immunol. 184: 6359-66.

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
NB600-336

c-Myc Epitope Tag (EQKLISEEDL) antibody

Western Blot: c-Myc Antibody - c-Myc Tag [NB600-336] - Western Blot analysis of c-Myc on Jurkat whole cell extract using NB600-336. Rabbit Aff - Purified Hu, Ms E, ICC/IF, IP, P, WB
0.1 ml / €340.00
    Novus Biologicals Inc.
+2 additional images
 
GTX20032

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse 9E10 Aff - Purified Hu C, E, EM, F, FN, IP, P
0.1 mg / €270.00
  IgG1   GeneTex Inc.
 
DB098

c-Myc Epitope Tag (EQKLISEEDL) antibody

c-Myc Epitope Tag (EQKLISEEDL) Mouse 9E10 Aff - Purified Hu C, IP, P, WB
0.1 mg / €310.00
  IgG1   Delta Biolabs LLC
 

3 Item(s)

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Antibody type
  1. Antibody (Ab) (3)
Clonality
  1. Monoclonal (2)
  2. Polyclonal (1)
Target
  1. c-Myc Epitope Tag (EQKLISEEDL) (3)
Applications
  1. Immunoprecipitation (IP) (3)
  2. Paraffin Sections (P) (3)
  3. Frozen sections (C) (2)
  4. Enzyme Immunoassay (E) (2)
  5. Western blot / Immunoblot (WB) (2)
  6. Immunoelectron microscopy (EM) (1)
  7. Flow cytometry (F) (1)
  8. Functional assay (FN) (1)
  9. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Host
  1. Mouse (2)
  2. Rabbit (1)
Images
  1. 1 image (1)
  2. 3+ images (1)
Images: Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  2. Western blot / Immunoblot (WB) (1)
Manufacturer
  1. Delta Biolabs LLC (1)
  2. GeneTex Inc. (1)
  3. Novus Biologicals Inc. (1)
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