c-Myc Epitope Tag (EQKLISEEDL)
Principal name
c-Myc Epitope Tag (EQKLISEEDL) antibody
Selected pictures
Gene ID
4609 (MYC)
Ncbi ID
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Functional assay (FN), Immunoelectron Microscopy (EM)
Background of c-Myc Epitope Tag (EQKLISEEDL) antibody
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. We offer anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Please visit our website at www.acris-antibodies.de for more informations.
General readings
1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. proto-oncogene product. Mol. Cell. Biol. 5: 3610 -3616.
2. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 7: 1299 -1304.
3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae. Microb. Cell Fact. 9: 74-86.
4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction. Biochem J. 404: 23-9.
5. Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J Cell Biol. 187: 959-66.
6. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 14: 803-6.
7. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins. 73: 552-65.
8. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling. J Immunol. 184: 6359-66.
Antibodies
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1182B |
c-Myc Epitope Tag (EQKLISEEDL) antibody | ||||||
![Anti-Myc epitope tag polyclonal antibody detects Myc-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-Myc epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 ug of recombinant protein containing the Myc epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/0163873.jpeg) |
Rabbit | Biotin | C, E, WB |
0.1 mg /
€320.00
|
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| Acris Antibodies GmbH | |||||||
| SM1863B |
c-Myc Epitope Tag (EQKLISEEDL) antibody | ||||||
 |
Mouse | 9E10 | Biotin | C, E, F, P, WB |
0.1 mg /
€430.00
|
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| IgG1 | Acris Antibodies GmbH | ||||||
| NB600-732 |
c-Myc Epitope Tag (EQKLISEEDL) antibody | ||||||
|
Mouse | 9E10 | Biotin | Hu | C, E, P, WB |
Regular Price: 50 µg / €310.00 Special Price: 50 µg / €250.00 |
|
| IgG1 | Novus Biologicals Inc. | ||||||
| GTX10825 |
c-Myc Epitope Tag (EQKLISEEDL) antibody | ||||||
|
Mouse | 9E10 | Biotin | Hu | C, E, P, WB |
50 µg /
€300.00
|
|
| IgG | GeneTex Inc. | ||||||
