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DYKDDDDK Epitope Tag

Principal name

DYKDDDDK Epitope Tag antibody

Selected pictures

SP5388P - Figure 1. Western blot of recombinant protein containing the epitope tag sequence DYKDDDDK using SP5388P.

SM3146P - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Figure 1A: Fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594).

Available reactivities

All Species, Mam (Mammalia)

Available hosts

Rabbit, Mouse, Chicken, Goat, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F)

Background of DYKDDDDK Epitope Tag antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK.

General readings

1. Chubet, R.G. and Brizzard, B.L. (1996) Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 20(1):136-141.
2. Slootstra, J.W. et al., (1997) Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. Mol Divers 2(3):156-164.
3. Robeva, A.S. et al., (1996) Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure. J. Biochem. Pharmacol. 51:4, 545-55.
4. Fulton, J.E. et al., (1995) Functional analysis of avian class I (BFIV) glycoproteins by epitope tagging and mutagenesis in vitro. Eur. J. Immunol. 25:7, 2069-76.