HA Epitope Tag (YPYDVPDYA)

Principal name

HA Epitope Tag (YPYDVPDYA) antibody

Selected pictures

HA Epitope Tag (YPYDVPDYA) antibody - NB600-363 HA Epitope Tag (YPYDVPDYA)
NB600-363 HA Epitope Tag (YPYDVPDYA) - Immunoprecipitation: HA Epitope Tag Antibody [NB600-363]- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.
HA Epitope Tag (YPYDVPDYA) antibody - NB600-363 HA Epitope Tag (YPYDVPDYA)
NB600-363 HA Epitope Tag (YPYDVPDYA) - Immunofluorescent: HA tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.

Available reactivities

Hu (Human), All Species, Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Chicken, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of HA Epitope Tag (YPYDVPDYA) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

General readings

(Conjugation) Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.

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Antibodies

Catalog No. Host Clone/Iso. Pres. React. Applications  
AP05575PU-N

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Purified E, P, WB
50 µg / €400.00
  IgG   Acris Antibodies GmbH
 
AP05576PU-N

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Purified E, P, WB
0.1 mg / €520.00
  IgG   Acris Antibodies GmbH
 
AP09230BT-N

HA Epitope Tag (YPYDVPDYA) antibody

Anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by western blot. Polyclonal rabbit-anti-HA epitope tag at a 1:10,000 dilution was used to detect 1.0 µg of recombinant transcription
factor protein containing the HA epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:4000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Biotin E, IP, P, WB
0.1 mg / €340.00
  IgG   Acris Antibodies GmbH
 
AP09230HR-N

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, P, WB
0.1 mg / €340.00
  IgG   Acris Antibodies GmbH
 
AP09230PU-N

HA Epitope Tag (YPYDVPDYA) antibody

Western blot: Anti-HA epitope tag antibody detects HA-tagged recombinant proteins. Polyclonal Rabbit anti-HA epitope tag, at a 1/2,000 dilution, was used to detect 1.0 µg of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye(R) 800 conjugated Gt-anti-Rabbit IgG (H&L) MX10 for 30 min at RT. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Aff - Purified E, P, WB
0.1 mg / €270.00
  IgG   Acris Antibodies GmbH
 
AP11018PU-N

HA Epitope Tag (YPYDVPDYA) antibody

figure 1. Western blot analysis of anti-HA tag Pab (Cat. #AP11018PU-N)in HA-tagged recombinant protein bacterial lysate.
Rabbit Purified E, WB
0.4 ml / €330.00
  Ig   Acris Antibodies GmbH
 
NB600-363

HA Epitope Tag (YPYDVPDYA) antibody

Immunoprecipitation: HA Epitope Tag Antibody [NB600-363]- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha. Rabbit Aff - Purified E, ICC/IF, IP, WB
0.1 mg / €290.00
    Novus Biologicals Inc.
 
NB600-366

HA Epitope Tag (YPYDVPDYA) antibody

Immunofluorescence: HA Epitope Tag Antibody [NB600-366]-CHO cell Rabbit Aff - Purified E, ICC/IF, IP, WB
1 mg / €940.00
    Novus Biologicals Inc.
 
NB600-391

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, ICC/IF, WB
0.1 mg / €340.00
    Novus Biologicals Inc.
 
GTX21208

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit FITC E, WB
0.1 mg / €300.00
  IgG   GeneTex Inc.
 
GTX29110

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Ig Fraction E, ICC/IF, IP, WB
0.1 mg / €300.00
  IgG   GeneTex Inc.
 
GTX30546

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit E, ICC/IF, WB
0.1 mg / €360.00
    GeneTex Inc.
 
GTX30574

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, ICC/IF, WB
0.1 mg / €420.00
    GeneTex Inc.
 
GTX77400

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, WB
0.1 mg / €350.00
    GeneTex Inc.
 
GTX77401

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Biotin E, WB
0.1 mg / €300.00
    GeneTex Inc.
 
PAB10343

HA Epitope Tag (YPYDVPDYA) antibody

HA-tag polyclonal antibody ( Cat # PAB10343 ) detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue.
Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta.
Sections of 4 µm were prepared from representative paraffin blocks.
HA-tag polyclonal antibody ( Cat # PAB10343 ) was diluted at 1 : 2,000.
Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada. Rabbit Hu E, ICC/IF, IP, P, WB
0.1 mg / €330.00
  IgG   Abnova Taiwan Corp.
 
PAB13749

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit E, E(capture), E(detection), ICC/IF, WB
0.1 mg / €340.00
  IgG   Abnova Taiwan Corp.
 
PAB13963

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit E, E(capture), ICC/IF, WB
0.1 mg / €330.00
  IgG   Abnova Taiwan Corp.
 
PG-10010

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Aff - Purified Hu E, IP, WB
0.1 mg / €320.00
    Genesis Biotech Inc.
 
18850

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Aff - Purified C, E, ICC/IF, WB
1 mg / €920.00
    QED Bioscience Inc.
 

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