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Home » Tag Antibodies » Epitope Tags » HA Epitope Tag (YPYDVPDYA)

HA Epitope Tag (YPYDVPDYA)

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Principal name

HA Epitope Tag (YPYDVPDYA) antibody

Selected pictures

HA Epitope Tag (YPYDVPDYA) antibody - NB600-363 HA Epitope Tag (YPYDVPDYA)
NB600-363 HA Epitope Tag (YPYDVPDYA) - Immunoprecipitation: HA Epitope Tag Antibody [NB600-363]- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.
HA Epitope Tag (YPYDVPDYA) antibody - NB600-363 HA Epitope Tag (YPYDVPDYA)
NB600-363 HA Epitope Tag (YPYDVPDYA) - Immunofluorescent: HA tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.

Available reactivities

Hu (Human), All Species, Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Chicken, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Dot blot (Dot), Paraffin Sections (P), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of HA Epitope Tag (YPYDVPDYA) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

General readings

1. Wu, et al. Mol. Biol. Cell 16(4): 1661-1672, 2005
2. Tanaka, et al. FEBS Lett. 579(7): 1579-1586, 2005.
3. Battacharya & Cabral. Mol. Biol. Cell 15(7): 3123-3131, 2004.
4. Yamanaka, et al. Curr. Biol. 13(9): 734-743, 2003.
5. Chang, et al. Genes Dev. 18(16): 2010-2023, 2004.
6. Su, et al. J. Immunological Methods. 291(12): 123-135, 2004.
7. Bruzzaniti, et al. Mol. Bioo. Cell 16(7): 3301-3313, 2005. Western Blot
8. Wang & Cabral. Biochim. Biophsy. Acta. 1744(2):245-255, 2005. 1. Glater, E. E., Megeath, L. J., Stowers, R. S., and Schwarz, T. L. (2006) Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent, 173, 545-557.
2. Wiesenthal, V., Leutz, A., and Calkhoven, C. F. (2006) A translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell, 34, e23-.

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AP00533PU-N

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Purified IP, WB
0.5 mg / €410.00
    Acris Antibodies GmbH
 
AP05575PU-N

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Purified E, P, WB
50 µg / €390.00
  IgG   Acris Antibodies GmbH
 
AP05576PU-N

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Purified E, P, WB
0.1 mg / €500.00
  IgG   Acris Antibodies GmbH
 
AP08109PU-N

HA Epitope Tag (YPYDVPDYA) antibody

Figure 1. Western blot analysis of extracts from 293 cells transfected with HA-tagged Akt using HA-Tag Polyclonal Antibody AP08109PU. Rabbit Aff - Purified All WB
0.1 mg / €280.00
    Acris Antibodies GmbH
 
AP09230BT-N

HA Epitope Tag (YPYDVPDYA) antibody

Anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by western blot. Polyclonal rabbit-anti-HA epitope tag at a 1:10,000 dilution was used to detect 1.0 µg of recombinant transcription factor protein containing the HA epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:4000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Biotin E, IP, P, WB
0.1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09230HR-N

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, P, WB
0.1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09230PU-N

HA Epitope Tag (YPYDVPDYA) antibody

Western blot: Anti-HA epitope tag antibody detects HA-tagged recombinant proteins. Polyclonal Rabbit anti-HA epitope tag, at a 1/2,000 dilution, was used to detect 1.0 µg of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye(R) 800 conjugated Gt-anti-Rabbit IgG (H&L) MX10 for 30 min at RT. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit Aff - Purified E, P, WB
0.1 mg / €250.00
  IgG   Acris Antibodies GmbH
+1 additional image
 
AP11018PU-N

HA Epitope Tag (YPYDVPDYA) antibody

figure 1. Western blot analysis of anti-HA tag Pab (Cat. #AP11018PU-N)in HA-tagged recombinant protein bacterial lysate. Rabbit Purified E, WB
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
 
SP5094P

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Aff - Purified WB
0.3 ml / €420.00
    Acris Antibodies GmbH
 
NB600-363

HA Epitope Tag (YPYDVPDYA) antibody

Immunoprecipitation: HA Epitope Tag Antibody [NB600-363]- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha. Rabbit Aff - Purified E, ICC/IF, IP, WB
0.1 mg / €290.00
    Novus Biologicals Inc.
+2 additional images
 
NB600-366

HA Epitope Tag (YPYDVPDYA) antibody

Immunofluorescence: HA Epitope Tag Antibody [NB600-366]-CHO cell Rabbit Aff - Purified E, ICC/IF, IP, WB
1 mg / €790.00
    Novus Biologicals Inc.
+2 additional images
 
NB600-391

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, ICC/IF, WB
0.1 mg / €310.00
    Novus Biologicals Inc.
 
GTX21208

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit FITC E, WB
0.1 mg / €300.00
  IgG   GeneTex Inc.
 
GTX29110

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Ig Fraction E, ICC/IF, IP, WB
0.1 mg / €260.00
  IgG   GeneTex Inc.
 
GTX30546

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit E, ICC/IF, WB
0.1 mg / €360.00
    GeneTex Inc.
 
GTX30574

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, ICC/IF, WB
0.1 mg / €390.00
    GeneTex Inc.
 
GTX73046

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Purified IP, WB
0.5 ml / €300.00
    GeneTex Inc.
 
GTX77399

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Aff - Purified E, WB
0.1 mg / €310.00
    GeneTex Inc.
 
GTX77400

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit HRP E, WB
0.1 mg / €330.00
    GeneTex Inc.
 
GTX77401

HA Epitope Tag (YPYDVPDYA) antibody

HA Epitope Tag (YPYDVPDYA) Rabbit Biotin E, WB
0.1 mg / €300.00
    GeneTex Inc.
 

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