HA Epitope Tag (YPYDVPDYA)

Principal name

HA Epitope Tag (YPYDVPDYA) antibody

Selected pictures

HA Epitope Tag (YPYDVPDYA) antibody - NB600-363 HA Epitope Tag (YPYDVPDYA)
NB600-363 HA Epitope Tag (YPYDVPDYA) - Immunoprecipitation: HA Epitope Tag Antibody [NB600-363]- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.
HA Epitope Tag (YPYDVPDYA) antibody - NB600-363 HA Epitope Tag (YPYDVPDYA)
NB600-363 HA Epitope Tag (YPYDVPDYA) - Immunofluorescent: HA tag Antibody [NB600-363] - Detection of HA-tagged Tubulin in CHO cells transfected with mutant beta-tubulin cDNA encoding an HA epitope tag. Photo contributed by R. Bhattacharya and F. Cabral, University of Texas Medical School at Houston.

Available reactivities

Hu (Human), All Species, Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Chicken, Goat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of HA Epitope Tag (YPYDVPDYA) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

General readings

(Conjugation) Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.

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