Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Principal name
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody
Selected pictures


Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Radioimmunoassay (R), Flow Cytometry (F), Paraffin Sections (P), Neutralisation (NEUT)
Background of Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
General readings
1. Southern, J.A. et al. (1991) Identification of an epitope on the P & V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72: 1551-1557.
2. Hanke, T. et al. (1992) Construction of solid matrix- antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73: 653-660.
3. Randall, R.E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrixantibody antigen (SMAA) complexes as vaccines. Vaccine 11: 1247-1252.
4. Randall, R.E. et al. (1994) Purification of antibody- antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. Vaccine 12: 351-358.
5. Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J. Virol. Methods 53:149-156.
6. Jaffray, E. et al. (1995) Domain structure of IkBa and sites of interaction with NF-kB p65. Mol. Cell. Biol. 15: 2166-2172.
7. Rodriguez, M.S. et al. (1995) Inducible degradation of IkB in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol. Cell. Biol. 15: 2413-2419.
8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696.
9. Hirst, K. et al. (1994) The transcription factor, CDK, its cyclin and their regulator; directing the transcription response to a nutritional signal. EMBO J. 13: 5410-5420.
10. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J. Immunol. Methods. 244: 141-150.
11. Young, D.F. et al. (2001) Single amino acid substitution in the protein of simian virus 5 differentitates its ability to block interferon signaling in human and murine cells. J. Virol. 75 (7): 3363 - 3370.
12. Stuart A. MacNeill (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the Bsubunit Nucleic Acids Research 32: 3005-3016.
13. Liebau, M.C. et al. (2011) Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia. J Biol Chem. Feb 28. [Epub ahead of print]
Antibodies
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| AM09145PU-N |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | V5.E3 | Purified | E, ICC/IF, WB |
0.1 mg /
€330.00
|
||
| IgG1 | Acris Antibodies GmbH | ||||||
| AP09228PU-N |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Rabbit | Aff - Purified | E, WB |
0.1 mg /
€250.00
|
|||
| IgG | Acris Antibodies GmbH | ||||||
| AP11020PU-N |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Rabbit | Purified | E, WB |
0.1 mg /
€330.00
|
|||
| Ig | Acris Antibodies GmbH | ||||||
+1 additional image | |||||||
| SM1691 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-PK1 | Purified | C, E, F, ICC/IF, IP, R, WB |
1 mg /
€550.00
|
||
| IgG2a | Acris Antibodies GmbH | ||||||
+1 additional image | |||||||
| SM1691B |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-PK1 | Biotin | C, E, WB |
0.1 mg /
€480.00
|
||
| IgG2a | Acris Antibodies GmbH | ||||||
| SM1691F |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-PK1 | FITC | ICC/IF |
0.1 mg /
€470.00
|
||
| IgG2a | Acris Antibodies GmbH | ||||||
| SM1691HRP |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-Pk1 | HRP | E, WB |
0.1 mg /
€590.00
|
||
| IgG2a | Acris Antibodies GmbH | ||||||
| SM1691PS |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-PK1 | Purified | C, E, ICC/IF, IP, R, WB |
0.1 mg /
€250.00
|
||
| IgG2a | Acris Antibodies GmbH | ||||||
+1 additional image | |||||||
| SM1691X |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-Pk1 | Purified | C, E, IP, R, WB |
2 mg /
€1,200.00
|
||
| IgG2a | Acris Antibodies GmbH | ||||||
| NB100-62264 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-Pk1 | Aff - Purified | Vi | C, E, F, ICC/IF, IP, P, R, WB |
Regular Price: 0.1 mg / €310.00 Special Price: 0.1 mg / €250.00 |
|
| IgG2a | Novus Biologicals Inc. | ||||||
| NB100-64791 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-Pk1 | Biotin | C, E, P, WB |
0.1 mg /
€430.00
|
||
| IgG2a | Novus Biologicals Inc. | ||||||
| NB600-379 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Chicken | Aff - Purified | E, ICC/IF, IP, WB |
0.1 mg /
€310.00
|
|||
| Novus Biologicals Inc. | |||||||
| NB600-380 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Goat | Aff - Purified | E, ICC/IF, IP, WB |
0.1 mg /
€290.00
|
|||
| Novus Biologicals Inc. | |||||||
| NB600-381 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
![]() |
Rabbit | Aff - Purified | E, ICC/IF, IP, WB |
0.1 ml /
€290.00
|
|||
| Novus Biologicals Inc. | |||||||
| NB600-386 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
![]() |
Goat | HRP | E, ICC/IF, WB |
0.1 mg /
€310.00
|
|||
| Novus Biologicals Inc. | |||||||
| NB600-387 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Goat | Agarose | IP |
0.1 mg /
€310.00
|
|||
| Novus Biologicals Inc. | |||||||
| NB600-395 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
![]() |
Rabbit | HRP | E, ICC/IF, WB |
0.1 mg /
€310.00
|
|||
| Novus Biologicals Inc. | |||||||
| NB600-695 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
![]() |
Mouse | SV5-PK1 | FITC | Vi | ICC/IF |
0.1 mg /
€430.00
|
|
| IgG2a | Novus Biologicals Inc. | ||||||
| NB600-849PEP |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) | ||||||
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E, NEUT, WB |
50 µl /
€230.00
|
|||||
| Novus Biologicals Inc. | |||||||
| NBP1-21645 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
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Mouse | SV5-Pk2 | Purified | E, IP, WB |
0.1 mg /
€330.00
|
||
| IgG1 | Novus Biologicals Inc. | ||||||

![Western blot using V5 tag antibody [V5.E3]: Western blot analysis of 293 cells transfected with V5-tagged vector (Lane 2) and untransfected control (Lane 1).](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/0166576.jpeg)
![Anti-V5 EPITOPE TAG polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar
results.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/0112251.png)

+1 additional image

![Western Blot: V5-TAG Antibody [NB100-62264] - V5 tagged protein detected with Mouse anti V5-tag](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/100-62264.jpg)
![Western Blot: V5 Epitope Tag Antibody (SV5-Pk1) [Biotin] [NB100-64791]- V5 tagged protein detected with Mouse anti V5-tag](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/37130_1.jpg)
![Western Blot: V5 Antibody [NB600-381] - Western blot analysis of V5-tagged Nicastrin, using NB600-381. Samples: NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 mcg/ml (1:5000).](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/p_43e25617900be.jpg)
![Western Blot: V5-TAG Antibody [NBP1-21645] - V5 tagged protein detected with Mouse anti V5-tag](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/NBP1-21645.jpg)