Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Principal name

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Selected pictures

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody - NB600-381 Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
NB600-381 Pk (V5) Epitope Tag (GKPIPNPLLGLDST) - Western Blot: V5 Antibody [NB600-381] - Western blot analysis of V5-tagged Nicastrin, using NB600-381. Samples: NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 mcg/ml (1:5000).
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody - SM1691
SM1691 - V5 tagged protein detected with Mouse anti V5-Tag (SM1691)

Available reactivities

Vi (Virus)

Available hosts

Rabbit, Chicken, Goat, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Radioimmunoassay (R), Flow Cytometry (F), Paraffin Sections (P), Neutralisation (NEUT)

Background of Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.

General readings

1. Southern, J.A. et al. (1991) Identification of an epitope on the P & V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72: 1551-1557.
2. Hanke, T. et al. (1992) Construction of solid matrix- antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73: 653-660.
3. Randall, R.E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrixantibody antigen (SMAA) complexes as vaccines. Vaccine 11: 1247-1252.
4. Randall, R.E. et al. (1994) Purification of antibody- antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. Vaccine 12: 351-358.
5. Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J. Virol. Methods 53:149-156.
6. Jaffray, E. et al. (1995) Domain structure of IkBa and sites of interaction with NF-kB p65. Mol. Cell. Biol. 15: 2166-2172.
7. Rodriguez, M.S. et al. (1995) Inducible degradation of IkB in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol. Cell. Biol. 15: 2413-2419.
8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696.
9. Hirst, K. et al. (1994) The transcription factor, CDK, its cyclin and their regulator; directing the transcription response to a nutritional signal. EMBO J. 13: 5410-5420.
10. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J. Immunol. Methods. 244: 141-150.
11. Young, D.F. et al. (2001) Single amino acid substitution in the protein of simian virus 5 differentitates its ability to block interferon signaling in human and murine cells. J. Virol. 75 (7): 3363 - 3370.
12. Stuart A. MacNeill (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the Bsubunit Nucleic Acids Research 32: 3005-3016.
13. Liebau, M.C. et al. (2011) Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia. J Biol Chem. Feb 28. [Epub ahead of print]

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42 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AM09145PU-N

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western blot using V5 tag antibody [V5.E3]: Western blot analysis of 293 cells transfected with V5-tagged vector (Lane 2) and untransfected control (Lane 1). Mouse V5.E3 Purified E, ICC/IF, WB
0.1 mg / €330.00
  IgG1   Acris Antibodies GmbH
 
AP09228PU-N

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Anti-V5 EPITOPE TAG polyclonal antibody detects V5-tagged recombinant protein by western blot. This antibody was used at 1.0 μg/ml to detect 0.05 μg (lane 2) of full-length recombinant mouse serum albumin containing the V5 epitope tag at the carboxy end. Comparison to MW markers (lane 1) indicates detection of monomeric V5 tagged albumin. A 4-20% gradient gel was used to separate the protein by SDS-PAGE under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar
results. Rabbit Aff - Purified E, WB
0.1 mg / €250.00
  IgG   Acris Antibodies GmbH
 
AP11020PU-N

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Figure 1. Western blot analysis of anti-V5 Tag Antibody (Cat.#AP11020PU-N) in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.
Rabbit Purified E, WB
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
 
SM1691

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

V5 tagged protein detected with Mouse anti V5-Tag (SM1691) Mouse SV5-PK1 Purified C, E, F, ICC/IF, IP, R, WB
1 mg / €550.00
  IgG2a   Acris Antibodies GmbH
 
SM1691B

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse SV5-PK1 Biotin C, E, WB
0.1 mg / €480.00
  IgG2a   Acris Antibodies GmbH
 
SM1691F

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse SV5-PK1 FITC ICC/IF
0.1 mg / €470.00
  IgG2a   Acris Antibodies GmbH
 
SM1691HRP

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse SV5-Pk1 HRP E, WB
0.1 mg / €590.00
  IgG2a   Acris Antibodies GmbH
 
SM1691PS

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

V5 tagged protein detected with Mouse anti V5-Tag (SM1691) Mouse SV5-PK1 Purified C, E, ICC/IF, IP, R, WB
0.1 mg / €250.00
  IgG2a   Acris Antibodies GmbH
 
SM1691X

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse SV5-Pk1 Purified C, E, IP, R, WB
2 mg / €1,200.00
  IgG2a   Acris Antibodies GmbH
 
NB100-62264

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western Blot: V5-TAG Antibody [NB100-62264] - V5 tagged protein detected with Mouse anti V5-tag Mouse SV5-Pk1 Aff - Purified Vi C, E, F, ICC/IF, IP, P, R, WB

Regular Price: 0.1 mg / €310.00

Special Price: 0.1 mg / €250.00

  IgG2a   Novus Biologicals Inc.
 
NB100-64791

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western Blot: V5 Epitope Tag Antibody (SV5-Pk1) [Biotin] [NB100-64791]- V5 tagged protein detected with Mouse anti V5-tag Mouse SV5-Pk1 Biotin C, E, P, WB
0.1 mg / €430.00
  IgG2a   Novus Biologicals Inc.
 
NB600-379

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Chicken Aff - Purified E, ICC/IF, IP, WB
0.1 mg / €310.00
    Novus Biologicals Inc.
 
NB600-380

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Goat Aff - Purified E, ICC/IF, IP, WB
0.1 mg / €290.00
    Novus Biologicals Inc.
 
NB600-381

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western Blot: V5 Antibody [NB600-381] - Western blot analysis of V5-tagged Nicastrin, using NB600-381. Samples: NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2).  NB600-381 was used at 0.2 mcg/ml (1:5000). Rabbit Aff - Purified E, ICC/IF, IP, WB
0.1 ml / €290.00
    Novus Biologicals Inc.
 
NB600-386

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Goat HRP E, ICC/IF, WB
0.1 mg / €310.00
    Novus Biologicals Inc.
 
NB600-387

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Goat Agarose IP
0.1 mg / €310.00
    Novus Biologicals Inc.
 
NB600-395

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Rabbit HRP E, ICC/IF, WB
0.1 mg / €310.00
    Novus Biologicals Inc.
 
NB600-695

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse SV5-PK1 FITC Vi ICC/IF
0.1 mg / €430.00
  IgG2a   Novus Biologicals Inc.
 
NB600-849PEP

Pk (V5) Epitope Tag (GKPIPNPLLGLDST)

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) E, NEUT, WB
50 µl / €230.00
    Novus Biologicals Inc.
 
NBP1-21645

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody

Western Blot: V5-TAG Antibody [NBP1-21645] - V5 tagged protein detected with Mouse anti V5-tag Mouse SV5-Pk2 Purified E, IP, WB
0.1 mg / €330.00
  IgG1   Novus Biologicals Inc.
 

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