Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Principal name
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody
Selected pictures


Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Radioimmunoassay (R), Flow Cytometry (F), Paraffin Sections (P), Neutralisation (NEUT)
Background of Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
General readings
1. Southern, J.A. et al. (1991) Identification of an epitope on the P & V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72: 1551-1557.
2. Hanke, T. et al. (1992) Construction of solid matrix- antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73: 653-660.
3. Randall, R.E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrixantibody antigen (SMAA) complexes as vaccines. Vaccine 11: 1247-1252.
4. Randall, R.E. et al. (1994) Purification of antibody- antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. Vaccine 12: 351-358.
5. Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding. J. Virol. Methods 53:149-156.
6. Jaffray, E. et al. (1995) Domain structure of IkBa and sites of interaction with NF-kB p65. Mol. Cell. Biol. 15: 2166-2172.
7. Rodriguez, M.S. et al. (1995) Inducible degradation of IkB in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol. Cell. Biol. 15: 2413-2419.
8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696.
9. Hirst, K. et al. (1994) The transcription factor, CDK, its cyclin and their regulator; directing the transcription response to a nutritional signal. EMBO J. 13: 5410-5420.
10. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J. Immunol. Methods. 244: 141-150.
11. Young, D.F. et al. (2001) Single amino acid substitution in the protein of simian virus 5 differentitates its ability to block interferon signaling in human and murine cells. J. Virol. 75 (7): 3363 - 3370.
12. Stuart A. MacNeill (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the Bsubunit Nucleic Acids Research 32: 3005-3016.
13. Liebau, M.C. et al. (2011) Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia. J Biol Chem. Feb 28. [Epub ahead of print]
Antibodies
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| NBP1-21645 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
![]() |
Mouse | SV5-Pk2 | Purified | E, IP, WB |
0.1 mg /
€330.00
|
||
| IgG1 | Novus Biologicals Inc. | ||||||
| NBP1-21646 |
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) antibody | ||||||
![]() |
Mouse | Purified | E, IP, WB |
0.1 mg /
€330.00
|
|||
| IgG1 | Novus Biologicals Inc. | ||||||

![Western Blot: V5-TAG Antibody [NBP1-21645] - V5 tagged protein detected with Mouse anti V5-tag](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/NBP1-21645.jpg)
![Western Blot: V5-TAG Antibody [NBP1-21646] - V5 tagged protein detected with Mouse anti V5-tag](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/NBP1-21646.jpg)