GFP

Principal name

GFP antibody

Selected pictures

GFP antibody - SP3005P
SP3005P - Figure 1. Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom). The natural fluorescence of the produced proteins is shown in the green channel (left), the anti-GFP antibody signal was detected in the red channel (right). The system was carefully tested for overlap of these two optical channels and images were scanned separately in sequential scanning mode. The blue nuclear stain is also shown.
GFP antibody - SP3005P
SP3005P - Figure 2. Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 0.5% Triton X-100, inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a rabbit anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together with a sample of the cell lysate (lane 1) were separated on SDS-PAGE polyacrylamide gel and immunoblotted with the anti-GFP antibody. The positions of molecular weight markers in kDa are indicated at the left.

Gene ID

3020 (H3F3A)

Ncbi ID

6100, NP_002098

Available reactivities

All Species, A. victoria, Hu (Human), Hydra

Available hosts

Rabbit, Chicken, Goat, Mouse, Sheep

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP), In Situ Hybridization (ISH), Immunoelectron Microscopy (EM), Dot blot (Dot), Flow Cytometry (F)

Background of GFP antibody

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm).
GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.
GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.

General readings

1. Jinhyun Kim, et al. J Neurophysiol, Oct 2008; 100: 1835 - 1847.
2. Witola, William H., et al. J. Biol. Chem. 2006 Jul 28;281(30):21305-21311. (Applications: IF)
3. Ward, W. W., et al.(1980) Photochem. Photobiol. 31:611-613.
4. Chalfie, M., et al.(1994) Science 263:802-805.
5. Wu C, et al. (1997) Gene 190(1):157-62.
6. Wachter RM, et al. (1998) Structure 6:1267-77.
Product Citations:
1. Niren Kapoor, et al. Knockdown of ASIC1 and Epithelial Sodium Channel Subunits Inhibits Glioblastoma Whole Cell Current and Cell Migration J. Biol. Chem. 2009; 284:24526-24541.
2. Maier B, et al. The unique hypusine modification of eIF5A promotes islet β cell inflammation and dysfunction in mice . J Clin Invest. 2010 Jun 1;120(6):2156-70
3. Edlira B. Clark, et al. Proteolytic Cleavage of Human Acid-sensing Ion Channel 1 by the Serine Protease Matriptase. J Biol Chem. 2010 Jul 2.
4. Lorestani A, et al. A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis. PLoS One. 2010 Aug 19;5(8). pii: e12302.
5. Clark EB, et al. Proteolytic Cleavage of Human Acid-sensing Ion Channel 1 by the Serine Protease Matriptase. J Biol Chem. 2010 Aug 27;285(35):27130-43. .
6. Kapoor N, et al. Interaction of ASIC1 and ENaC Subunits in Human Glioma Cells and Rat Astrocytes. Am J Physiol Cell Physiol. 2011 Feb 23.
7. Witola, William H., et al. Localization of the Phosphoethanolamine Methyltransferase of the Human Malaria Parasite Plasmodium falciparum to the Golgi Apparatus J. Biol. Chem. 2006 Jul 28;281(30):21305-21311
8. Jinhyun Kim, et al. Kv4 Accessory Protein DPPX (DPP6) is a Critical Regulator of Membrane Excitability in Hippocampal CA1 Pyramidal Neurons J Neurophysiol, Oct 2008; 100: 1835-1847.

6 Item(s)

per page

Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
AP05978PU-N

GFP antibody

GFP Chicken IgG Purified C, IP, WB
0.25 mg / €560.00
    Acris Antibodies GmbH
AP09426PU-N

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of GFP protein detected with polyclonal anti-GFP antibody. Lane 1 shows negative control staining of 20 µg of mouse spleen lysate. Lane 2 shows staining of mouse spleen lysate spiked with 50 ng of wt GFP. This antibody detects a 27 kDa band corresponding to the GFP epitope tag commonly used in recombinant constructs. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE followed by transfer to nitrocellulose using standard methods. After blocking with 5% BSA in PBS, the membrane was probed for 2 h at room temperature with the primary antibody diluted in 5% BSA to 2 µg/ml, followed by washes and reaction with a 1:20,000 dilution of IRDye(TM)800 Conjugated Affinity
Purified anti-Chicken IgG [H&L] [Goat] MX10. The IRDye®(R)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Chicken Ig Aff - Purified A. victoria E, ICC/IF, WB
0.1 mg / €250.00
    Acris Antibodies GmbH
AP09426PU-S

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of GFP protein detected with polyclonal anti-GFP antibody. Lane 1 shows negative control staining of 20 µg of mouse spleen lysate. Lane 2 shows staining of mouse spleen lysate spiked with 50 ng of wt GFP. This antibody detects a 27 kDa band corresponding to the GFP epitope tag commonly used in recombinant constructs. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE followed by transfer to nitrocellulose using standard methods. After blocking with 5% BSA in PBS, the membrane was probed for 2 h at room temperature with the primary antibody diluted in 5% BSA to 2 µg/ml, followed by washes and reaction with a 1:20,000 dilution of IRDye(TM)800 Conjugated Affinity
Purified anti-Chicken IgG [H&L] [Goat] MX10. The IRDye®(R)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Chicken Ig Aff - Purified A. victoria E, ICC/IF, WB
25 µl / €190.00
    Acris Antibodies GmbH
AP09427PU-N

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Chicken Ig Aff - Purified A. victoria E, WB
0.3 mg / €410.00
    Acris Antibodies GmbH
NB100-1614

GFP antibody

GFP Chicken Aff - Purified ICC/IF, P, WB
0.1 ml / €290.00
    Novus Biologicals Inc.
GTX13970

GFP antibody

GFP Chicken Ig Fraction C, WB
50 µl / €290.00
    GeneTex Inc.

6 Item(s)

per page
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