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GST-Tag

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Principal name

GST-Tag antibody

Selected pictures

GST-Tag antibody - SM6024
SM6024 - Western blot analysis: Recombinant GST (28 kDa) and GST-fusion protein (61 kDa) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate GST and GST-fusion proteins (20 ng).
GST-Tag antibody

Gene ID

2950 (GSTP1)

Ncbi ID

6182, NP_000843.1

Available reactivities

Sj (Stapylococcus japonicum), E. coli (Escherichia coli), Mam (Mammalia), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoelectron Microscopy (EM), Flow Cytometry (F), Paraffin Sections (P), Dot blot (Dot), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of GST-Tag antibody

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant GST was expressed in E.coli and purified by conventional chromatography techniques.

General readings

This product has been used in:

Lehti K et al. Oligomerization through hemopexin and cytoplasmic domains regulates the activity and turnover of membrane-type 1 matrix metalloproteinase. J Biol Chem 277:8440-8 (2002).

Chen, P-L., Chen, C-F., Chen, Y., Xiao, J., Sharp, Z.D. and Lee, W-H. (1998) The BRC repeats in BRCA2 are Critical for RAD51 Binding and Resistance to Methyl Methanesulfonate Treatment. Proc Natl Acad Science, USA 95:5287-5292. 1998

Carlos M. Luque, Carmen M. Pérez-Ferreiro, Alicia Pérez-González, Ludwig Englmeier, Maria D. Koffa, and Isabel Correas. An Alternative Domain Containing a Leucine-rich Sequence Regulates Nuclear Cytoplasmic Localization of Protein 4.1R. J. Biol. Chem., Jan 2003; 278: 2686 - 2691. 2003

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
GTX19585

GSTP1 / GST3 antibody

GSTP1 / GST3 Mouse BDI340 Purified Ms, Rt E, IP, WB
50 µg / €450.00
  IgG1   GeneTex Inc.
 
AM00069PU-N

GST-Tag antibody

Antibody sensitivity Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 4H3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 100ng, lane 2: 50ng, lane 3: 25ng, lane 4: 10ng, lane 5: 5ng, lane 6: 2ng, lane 7: 1ng Mouse 4H3 Purified Sj E, IP, WB
0.1 mg / €340.00
  IgG2a   Acris Antibodies GmbH
 
AM00281PU-N

GST-Tag antibody

GST-Tag Mouse GST01 Purified Sj WB
0.1 mg / €420.00
  IgG1   Acris Antibodies GmbH
 
AM05392PU-N

GST-Tag antibody

Western Blot Mouse KAB29 Purified WB
0.1 mg / €300.00
  IgG1   Acris Antibodies GmbH
 
AM08430PU-N

GST-Tag antibody

Western blot using Affinity Purified anti-GST antibody to detect purified GST recombinant protein (Left) and in a lysate of HeLa cells expressing recombinant GST protein (Right). Approximately 0.1 μg of protein was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at RT followed by washes and reaction with a 1/5,000 dilution of IRDye(TM)800 conjugated Goat anti-Mouse IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Mouse 3D4 Purified C, E, WB
0.5 mg / €310.00
  IgG1   Acris Antibodies GmbH
 
AM09141PU-N

GST-Tag antibody

Recombinant GST protein (20ng, Lane 1) and 293 cellular lysates transfected with a GST expression vector (20µg, Lane 2) were probed with anti-GST (1µg/ml). Arrow indicates GST (~26kDa) and GST-fustion protein (~29kDa). Mouse GST.B6 Purified C, IP, WB
0.1 mg / €290.00
  IgG2a   Acris Antibodies GmbH
 
AM11012PU-N

GST-Tag antibody

Figure 1. Western blot using GST Mab (Cat. #AM11012PU-N) to detect GST recombinant protein purified from bacterial lysate. Mouse 9AT106 Purified E, WB

Regular Price: 0.1 mg / €330.00

Special Price: 0.1 mg / €231.00

  IgG1   Acris Antibodies GmbH
 
AP01064BT-N

GST-Tag antibody

GST-Tag Goat Biotin Sj E, WB
1 mg / €320.00
    Acris Antibodies GmbH
 
AP09105FC-N

GST-Tag antibody

GST-Tag Goat FITC C, Dot, E, F, IP, WB
20 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09112AG-N

GST-Tag antibody

GST-Tag Goat Agarose
20 mg / €270.00
  IgG   Acris Antibodies GmbH
 
AP09205AP-N

GST-Tag antibody

GST-Tag Goat AP Sj E, IP, WB
1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09205FC-N

GST-Tag antibody

GST-Tag Goat FITC Sj F, ICC/IF
1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09205HR-N

GST-Tag antibody

GST-Tag Goat HRP C, Dot, E, IP, WB
1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09205TC-N

GST-Tag antibody

GST-Tag Goat TRITC Sj F
1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09205TR-N

GST-Tag antibody

GST-Tag Goat Texas Red Sj E, IP, WB
1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP11290PU-N

GST-Tag antibody

Western Blot analysis using anti-GST Pab (Cat. #AP11290PU-N) to detect a GST-fusion recombinant protein (42 kDa) purified from bacterial lysate (Lanes 1-3: 10, 40, and 160 ng GST-fusion protein). Rabbit Purified Hu E, P, WB
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
 
R1088

GST-Tag antibody

GST-Tag Goat Serum Sj E, IP, WB
2 ml / €230.00
    Acris Antibodies GmbH
 
R1088P

GST-Tag antibody

GST-Tag Goat Ig Fraction Sj E, IP, WB
50 mg / €250.00
    Acris Antibodies GmbH
 
SM1690

GST-Tag antibody

GST-Tag Mouse vpg66 Supernatant E, WB
2 ml / €410.00
  IgG1   Acris Antibodies GmbH
 
SM1690B

GST-Tag antibody

Western blot analysis of GST ubiquitin recombinant protein probed with MOUSE ANTI GST:BIOTIN (SM1690B) followed by STREPTAVIDIN: HRP Mouse vpg66 Biotin E, WB
0.1 mg / €410.00
  IgG1   Acris Antibodies GmbH
 

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Antibody type
  1. Antibody (Ab) (72)
  2. Control peptide (Ab/CP) (1)
Clonality
  1. Polyclonal (48)
  2. Monoclonal (25)
Target
  1. GST-Tag (78)
  2. GSTP1 / GST3 (1)
Property
  1. 1-425 (1)
Reactivity
  1. Stapylococcus japonicum (Sj) (36)
  2. Human (Hu) (6)
  3. Mouse (Ms) (3)
  4. E. coli (1)
  5. Mammalia (Mam) (1)
  6. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (59)
  2. Enzyme Immunoassay (E) (44)
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  5. Frozen sections (C) (16)
  6. Flow cytometry (F) (5)
  7. Dot blot (Dot) (2)
  8. Paraffin Sections (P) (2)
  9. ELISA (capture) (E(capture)) (1)
  10. ELISA (detection) (E(detection)) (1)
  11. Immunoelectron microscopy (EM) (1)
Presentation
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  3. HRP (7)
  4. Agarose (5)
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  11. TRITC (1)
  12. Texas Red (1)
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  1. Goat (28)
  2. Mouse (24)
  3. Rabbit (18)
  4. Chicken (1)
Images
  1. 1 image (23)
Images: Applications
  1. Western blot / Immunoblot (WB) (18)
  2. SDS-PAGE (5)
Species (Protein)
  1. Schistosoma (4)
Source
  1. E. coli (4)
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  2. Abnova Taiwan Corp. (17)
  3. Novus Biologicals Inc. (14)
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