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RFP-Tag

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Principal name

RFP-Tag antibody

Selected pictures

RFP-Tag antibody - PAB11376
PAB11376 - Western blot of RFP recombinant protein detected with RFP polyclonal antibody ( Cat # PAB11376 ).Lane 1 shows no reaction against a GFP recombinant protein present in 10 µg of HeLa cell extract.Lane 2 shows a single band detected in 10 µg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band.A 4-12% Bis-Tris gradient gel ( Invitrogen ) was used for SDS-PAGE. The membrane was blocked and then probed with RFP polyclonal antibody ( Cat # PAB11376 ) diluted 1:2,500 for 1 h at RT.
RFP-Tag antibody - PAB9104
PAB9104 - Western blot analysis with recombinant RFP.Lane 1 : 10 ng.Lane 2 : 50 ng.Lane 3 : 250 ng.Lane 4 : 1 µg.

Ncbi ID

86600

Available hosts

Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of RFP-Tag antibody

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.

General readings

1. Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990 (Comnjugation).
2. Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY: The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA 2000, 97: 11990-11995.

6 Item(s)

per page

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AP09229BT-N

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Separation was achieved using a 4-12% Bis Tris gradient gel followed by transfer to nitrocellulose and blocking.The membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit Biotin C, E, WB
0.1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09229FC-N

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected withanti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDSPAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit FITC E, F, ICC/IF, WB
0.1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09229HR-N

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit HRP C, E, WB
0.1 mg / €320.00
  IgG   Acris Antibodies GmbH
 
AP09229PU-N

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit Aff - Purified E, P, WB
0.1 mg / €240.00
  IgG   Acris Antibodies GmbH
 
PAB9104

RFP-Tag antibody

Western blot analysis with recombinant RFP. Lane 1 : 10 ng. Lane 2 : 50 ng. Lane 3 : 250 ng. Lane 4 : 1 µg. Rabbit ICC/IF, IP, WB
0.1 mg / €350.00
  IgG   Abnova Taiwan Corp.
 
PAB11376

RFP-Tag antibody

Western blot of RFP recombinant protein detected with RFP polyclonal antibody ( Cat # PAB11376 ). Lane 1 shows no reaction against a GFP recombinant protein present in 10 µg of HeLa cell extract. Lane 2 shows a single band detected in 10 µg of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel ( Invitrogen ) was used for SDS-PAGE. The membrane was blocked and then probed with RFP polyclonal antibody ( Cat # PAB11376 ) diluted 1:2,500 for 1 h at RT. Rabbit C, E, ICC/IF, WB
0.1 mg / €330.00
  IgG   Abnova Taiwan Corp.
 

6 Item(s)

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Product Type
  1. Antibodies (6)
Antibody type
  1. Antibody (Ab) (6)
Clonality
  1. Polyclonal (6)
Target
  1. RFP-Tag (6)
Property
  1. Ads. to Hu (4)
  2. Ms (4)
  3. Rt Serum Proteins (4)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Enzyme Immunoassay (E) (5)
  3. Frozen sections (C) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  5. Flow cytometry (F) (1)
  6. Immunoprecipitation (IP) (1)
  7. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (1)
  2. Biotin (1)
  3. FITC (1)
  4. HRP (1)
Host
  1. Rabbit (6)
Images
  1. 1 image (6)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
Manufacturer
  1. Acris Antibodies GmbH (4)
  2. Abnova Taiwan Corp. (2)
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