14-3-3 protein epsilon antibody

Principal name

14-3-3 protein epsilon antibody

Alternative names for 14-3-3 protein epsilon antibody

14-3-3E, YWHAE, FocusOn116

Selected pictures

14-3-3 protein epsilon antibody - AP06766PU-N
AP06766PU-N - Western blot analysis of AP06766PU-N 14-3-3 ε antibody in extracts from HepG2 cells.
14-3-3 protein epsilon antibody - AP06766PU-N
AP06766PU-N - Immunohistochemistry analyzes of AP06766PU-N 14-3-3 ε antibody in Paraffin-Embedded Human brain tissue.

SwissProt ID

P62258 (Human), P62259 (Mouse), P62260 (Rat), P62261 (Bovin), P62262 (Sheep), Q5ZMT0 (Chick)

Gene ID

7531 (YWHAE), 22627 (Ywhae)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit, Chicken, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of 14-3-3 protein epsilon antibody

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, episilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

General readings

1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein. FEBS 331: 296-303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162-172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors. PLoS One. 6: e24843.

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AM09042PU-N

14-3-3 protein epsilon antibody

Western blot analysis: The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 epsilon (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse 5A5 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  IgG2a   Acris Antibodies GmbH
 
AM09042PU-S

14-3-3 protein epsilon antibody

Western blot analysis: The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 epsilon (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse 5A5 Purified Hu, Ms E, P, WB

Regular Price: 50 µl / €230.00

Special Price: 50 µl / €161.00

  IgG2a   Acris Antibodies GmbH
 
AP05565SU-N

14-3-3 protein epsilon (C-term) antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, P, WB
0.1 ml / €430.00
  IgG   Acris Antibodies GmbH
 
AP05568SU-N

14-3-3 protein epsilon antibody

Human Breast Epithelium stained with Rabbit anti 14-3-3 Epsilon antibody Cat.-No AP05568SU-N
Rabbit Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, P, WB
0.1 ml / €430.00
  IgG   Acris Antibodies GmbH
 
AP06766PU-N

14-3-3 protein epsilon antibody

Western blot analysis of AP06766PU-N 14-3-3 ε antibody in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
AP12543CP-N

14-3-3 protein epsilon control peptide

14-3-3 protein epsilon
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP12544CP-N

14-3-3 protein epsilon control peptide

14-3-3 protein epsilon
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP22883SU-N

14-3-3 protein epsilon (N-term) antibody

Human Breast (formalin-fixed, paraffin-embedded) stained with YWHAE antibody AP22883SU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Serum Sh E, P, WB
50 µl / €380.00
    Acris Antibodies GmbH
 
AP32388PU-N

14-3-3 protein epsilon pSer185 antibody

14-3-3 protein epsilon Sheep Aff - Purified Bov, Hu, Ms, Rb, Rt, Sh WB
0.1 ml / €340.00
  IgG   Acris Antibodies GmbH
 
NB100-92415

14-3-3 protein epsilon antibody

Western Blot: 14-3-3 episilon Antibody [NB100-92415] - Western blot (WB) analysis of 14-3-3 å (D238)  antibody in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  IgG   Novus Biologicals Inc.
 
NBP1-22960

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Mouse 5A5 Purified Hu E, SDS-PAGE, WB
0.1 ml / €340.00
  IgG2a   Novus Biologicals Inc.
 
NBP1-32695

14-3-3 protein epsilon antibody

Immunohistochemistry: 14-3-3 epsilon Antibody [NBP1-32695] - analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution. Rabbit Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  IgG   Novus Biologicals Inc.
 
NBP1-33554

14-3-3 protein epsilon antibody

Western  Blot:  14-3-3 epsilon Antibody [NBP1-33554] – Western blot analysis of 14-3-3 epsilon on H1299 lysates using NBP1-33554 at a dilution of 1:1000. 30ug whole cell lysate loaded on 12% SDS PAGE gel. Rabbit Hu ICC/IF, P, WB
0.1 ml / €390.00
  IgG   Novus Biologicals Inc.
 
NBP1-68205-0.1

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €340.00
  IgG   Novus Biologicals Inc.
 
NBP1-68205-50

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  IgG   Novus Biologicals Inc.
 
NBP1-89827

14-3-3 protein epsilon antibody

Western Blot: 14-3-3 epsilon Antibody [NBP1-89827] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil Rabbit Aff - Purified Hu C, ICC/IF, P, WB
0.1 ml / €360.00
  IgG   Novus Biologicals Inc.
 
GTX82193

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Chicken Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €460.00
    GeneTex Inc.
 
GTX109090

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €300.00
    GeneTex Inc.
 
GTX113576

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Rabbit Aff - Purified Hu WB
0.1 ml / €300.00
    GeneTex Inc.
 
H00007531-A01

14-3-3 protein epsilon antibody

14-3-3 protein epsilon Mouse Serum Hu E, WB
50 µl / €260.00
    Abnova Taiwan Corp.
 

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