14-3-3 protein eta antibody

Principal name

14-3-3 protein eta antibody

Alternative names for 14-3-3 protein eta antibody

Protein AS1, YWHAH, YWHA1, FocusOn116

Selected pictures

14-3-3 protein eta antibody - 15222-1-AP 14-3-3 protein eta
15222-1-AP 14-3-3 protein eta - Human brain were subjected to SDS PAGE followed by western blot with 15222-1-AP(YWHAH Antibody) at dilution of 1:1000
14-3-3 protein eta antibody - AP20467PU-N
AP20467PU-N - Western blot (WB) analysis of 14-3-3 theta pAb in extracts from Jurkat cells.

SwissProt ID

P68509 (Bovin), P68510 (Mouse), P68511 (Rat), Q04917 (Human)

Gene ID

7533 (YWHAH)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of 14-3-3 protein eta antibody

14-3-3 eta is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 eta is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alphasynuclein, forming part of lewy bodies in parkinsons disease and has been linked to earlyonset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL1/Protein 4.1B tumour suppressor. Furthermore the glucocortcoid receptor is positively regulated by 14-3-3eta.
AHP1046 may not react with recombinant proteins that are not Nacetylated.

General readings

1. Martin, H., et al., FEBS Journal (1993) 331:296-303.
2. Wheeler-Jones, C., et al., Biochem J (1996) 315:41-47.
3. Wiltfang, J., et al., Journal of Neurochemistry (1999) 73:2485-2490.
4. Baxter, H., et al., Neuroscience (2002) 109:5-14.
5. Chaudhri, M., et al., Biochem Biophys Res Comm (2003) 300:679-685.
6. Steinacker, P., et al., Molecular and Cellular Biology (2005) 25:1339-1346.

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
11648-2-AP

14-3-3 protein eta antibody

Immunofluorescent analysis of HepG2 cells, using 11648-2-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Rabbit Aff - Purified Hu, Ms E, P, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
 
15222-1-AP

14-3-3 protein eta antibody

Human brain were subjected to SDS PAGE followed by western blot with 15222-1-AP(YWHAH Antibody) at dilution of 1:1000 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
 
AP05566SU-N

14-3-3 protein eta antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh P, WB
0.1 ml / €430.00
  IgG   Acris Antibodies GmbH
 
AP17848CP-N

14-3-3 protein eta Control Peptide

14-3-3 protein eta
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP18246CP-N

14-3-3 protein eta Control Peptide

14-3-3 protein eta
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP20467PU-N

14-3-3 protein eta antibody

Western blot (WB) analysis of 14-3-3 theta pAb in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
AP22684SU-N

14-3-3 protein eta (N-term) antibody

Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with YWHAH antibody AP22684SU-N at 1/250 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh P, WB
50 µl / €380.00
  IgG   Acris Antibodies GmbH
 
AP32434SU-N

14-3-3 protein eta (N-term) antibody

14-3-3 protein eta Rabbit Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh C, WB
0.1 ml / €350.00
    Acris Antibodies GmbH
 
NB100-65809

14-3-3 protein eta antibody

WB: 14-3-3 ETA Antibody [NB100-65809] - WB of 14-3-3 isoforms in HEK293 cell extracts using AbD Serotec antibodies. Lane 1 = anti-gamma 14-3-3, anti-zeta 14-3-3(2), anti-C-epsilon-14-3-3(3), anti-N-epsilon-14-3-3(4), anti-eta 14-3-3(5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3. Rabbit Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh P, WB
0.1 ml / €460.00
  IgG   Novus Biologicals Inc.
 
GTX104758

14-3-3 protein eta antibody

14-3-3 protein eta Rabbit Aff - Purified Hu WB
0.1 ml / €300.00
    GeneTex Inc.
 
GTX113577

14-3-3 protein eta antibody

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX113577 diluted at 1:10000 Rabbit N1C3 Purified Hu WB
0.1 ml / €300.00
  IgG   GeneTex Inc.
 
H00007533-A01

14-3-3 protein eta antibody

YWHAH polyclonal antibody (A01), Lot # 060524JCS1 Western Blot analysis of YWHAH expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €260.00
    Abnova Taiwan Corp.
 
H00007533-M04

14-3-3 protein eta antibody

Detection limit for recombinant GST tagged YWHAH is approximately 0.1ng/ml as a capture antibody. Mouse 6A12 Purified Hu E, WB
0.1 mg / €340.00
  IgG2a   Abnova Taiwan Corp.
 
H00007533-M07

14-3-3 protein eta antibody

14-3-3 protein eta Mouse 1C2 Purified Hu E, WB
0.1 mg / €340.00
  IgG1   Abnova Taiwan Corp.
 
C12003-1

14-3-3 protein eta antibody

Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €195.00
    Assay Biotech
 
C12003-2

14-3-3 protein eta antibody

Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €290.00
    Assay Biotech
 

Proteins

Catalog No. Species Pres. Purity   Source  
AR09102PU-L

14-3-3 protein eta (1-246, His-tag)

14-3-3 eta, 1-246 aa, His-tagged: 15% SDS-PAGE (4 µg) Human Purified ≥ 95 E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH
 
AR09102PU-N

14-3-3 protein eta (1-246, His-tag)

14-3-3 eta, 1-246 aa, His-tagged: 15% SDS-PAGE (4 µg) Human Purified ≥ 95 E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH
 
H00007533-Q01-10

14-3-3 protein eta

14-3-3 protein eta Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
 
H00007533-Q01-25

14-3-3 protein eta

14-3-3 protein eta Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
 

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