14-3-3 protein gamma antibody

Principal name

14-3-3 protein gamma antibody

Alternative names for 14-3-3 protein gamma antibody

YWHAG, KCIP-1, Protein kinase C inhibitor protein 1

Selected pictures

14-3-3 protein gamma antibody - NB100-406 14-3-3 protein gamma
NB100-406 14-3-3 protein gamma - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).
14-3-3 protein gamma antibody - NB100-407 14-3-3 protein gamma
NB100-407 14-3-3 protein gamma - Immunocytochemistry: 14-3-3 gamma Antibody (HS23) [NB100-407] - Detection of 14-3-3 gamma (green) in HepG2 cells using NB100-407. Nuclei (Blue) are counterstained with Hoechst 33258.

SwissProt ID

P61981 (Human), P61982 (Mouse), P61983 (Rat), P68252 (Bovin), P68253 (Sheep), Q5F3W6 (Chick)

Gene ID

7532 (YWHAG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of 14-3-3 protein gamma antibody

14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 gamma is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds musclespecific receptor tyrosine kinase regulating synaptic gene transmission. The gamma isoform binds amyloid betaprotein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65dependent gene transactivation. The binding of 14-3-3 gamma to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.

General readings

1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.

  • Pinterest
  • LinkedIn