14-3-3 protein gamma antibody

Principal name

14-3-3 protein gamma antibody

Alternative names for 14-3-3 protein gamma antibody

YWHAG, KCIP-1, Protein kinase C inhibitor protein 1

Selected pictures

14-3-3 protein gamma antibody - AP20539PU-N 14-3-3 protein gamma
AP20539PU-N 14-3-3 protein gamma - Western blot analysis of 14-3-3 gamma Antibody (Cat.-No AP20539PU-N) in extracts from Hela cells at 1/500 dilution.
14-3-3 protein gamma antibody - AP20539PU-N
AP20539PU-N - Immunohistochemistry (IHC) analyzes of 14-3-3 gamma antibody (Cat.-No.: AP20539PU-N) in paraffin-embedded human brain tissue.

SwissProt ID

P61981 (Human), P61982 (Mouse), P61983 (Rat), P68252 (Bovin), P68253 (Sheep), Q5F3W6 (Chick)

Gene ID

7532 (YWHAG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of 14-3-3 protein gamma antibody

14-3-3 gamma is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 gamma is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds musclespecific receptor tyrosine kinase regulating synaptic gene transmission. The gamma isoform binds amyloid betaprotein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65dependent gene transactivation. The binding of 14-3-3 gamma to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.

General readings

1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
12381-1-AP

14-3-3 protein gamma antibody

RNAi result of 12381-1-AP(anti-14-3-3G) in Mouse IMCD3 cells From Dr.Seongjin Seo. Rabbit IgG Aff - Purified Hu, Ms, Rt E, IP, WB
0.15 ml / €250.00
  Proteintech Group, Inc.
AM09044PU-N

14-3-3 protein gamma antibody

Western blot analysis: Cell lysates of HeLa (30 ug) and mouse brain (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human 14-3-3 gamma antibody  (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b J3H10 Purified Hu, Ms E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH
AM09044PU-S

14-3-3 protein gamma antibody

Western blot analysis: Cell lysates of HeLa (30 ug) and mouse brain (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human 14-3-3 gamma antibody  (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b J3H10 Purified Hu, Ms E, WB
50 µl / €230.00
  Acris Antibodies GmbH
GTX110629

14-3-3 protein gamma antibody

Sample (30 ug of whole cell lysate)  A: Hep G2 (GTX27900)
12% SDS PAGE  GTX110629 diluted at 1:1000 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €320.00
  GeneTex Inc.
GTX113298

14-3-3 protein gamma antibody

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
12% SDS PAGE
GTX113298 diluted at 1:1000 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €320.00
  GeneTex Inc.
Sample size available for 25µl / 99€ (read more…)
H00007532-M02

14-3-3 protein gamma antibody

YWHAG monoclonal antibody (M02), clone 6A10. Western Blot analysis of YWHAG expression in Raw 264.7 ( Cat # L024V1 ). Mouse IgG2b 6A10 Purified Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Abnova Taiwan Corp.
AM26569AF-N

14-3-3 protein gamma (1-12) antibody

Immunoprecipitation of 14-3-3γ from A431 cells with mouse IgG2a (1) or AM26569AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26569AF-N. Mouse IgG2a KC21 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €290.00
  Acris Antibodies GmbH
AM32791PU-N

14-3-3 protein gamma (incl. pos. control) antibody

Western Blot: 3T3/A31 cell lysate probed with anti-14-3-3 gamma Antibody (0.05 μg/ml). Mouse CG31-2B6 Purified Hu, Ms, Rt WB
0.1 mg / €360.00
  Acris Antibodies GmbH
AM50336PU-N

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG1 AT4B9 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH
AM50336PU-S

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG1 AT4B9 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH
AP05567SU-N

14-3-3 protein gamma antibody

Western blots of 14-3-3 isoforms in HEK293 cell extracts using 14-3-3 antibodies.
Lane 1: anti-gamma 14-3-3 (AP05567SU-N)
Lane 2: anti-zeta 14-3-3 (AP05572SU-N)
Lane 3: anti-C-epsilon-14-3-3 (AP05565SU-N)
Lane 4: anti-N-epsilon-14-3-3 (AP05568SU-N)
Lane 5: anti-eta 14-3-3. (AP05566SU-N)

The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rt, Sh E, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH
AP17846CP-N

14-3-3 protein gamma Control Peptide

14-3-3 protein gamma
0.1 mg / €120.00
  Acris Antibodies GmbH
AP17846PU-N

14-3-3 protein gamma antibody

Western blot analysis of YWHAG Antibody (Center) (AP17846PU-N) in mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €340.00
  Acris Antibodies GmbH
AP17847CP-N

14-3-3 protein gamma Control Peptide

14-3-3 protein gamma
0.1 mg / €120.00
  Acris Antibodies GmbH
AP17847PU-N

14-3-3 protein gamma (N-term) antibody

Western blot analysis of YWHAG Antibody (N-term) (AP17847PU-N) in A375 cell line and mouse cerebellum tissue lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €340.00
  Acris Antibodies GmbH
AP20539PU-N

14-3-3 protein gamma antibody

Immunohistochemistry (IHC) analyzes of 14-3-3 gamma antibody (Cat.-No.: AP20539PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
NB100-406

14-3-3 protein gamma (N-term modified) antibody

14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa  cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Mouse IgG2a KC21 Ascites Hu WB
0.1 ml / €330.00
  Novus Biologicals Inc.
NB100-406SS

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG2a KC21 Hu WB
25 µl / €150.00
  Novus Biologicals Inc.
NB100-407

14-3-3 protein gamma (N-term modified) antibody

Immunocytochemistry: 14-3-3 gamma Antibody (HS23) [NB100-407] - Detection of 14-3-3 gamma (green) in HepG2 cells using NB100-407.  Nuclei (Blue) are counterstained with Hoechst 33258. Mouse IgG2a HS23 Ascites Hu WB
0.1 ml / €320.00
  Novus Biologicals Inc.
NB100-407SS

14-3-3 protein gamma antibody

14-3-3 protein gamma Mouse IgG1 HS23 Ascites Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, WB
25 µl / €170.00
  Novus Biologicals Inc.

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