Alternative names for 14-3-3 protein theta antibody
14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1
A0758-1 14-3-3 protein theta - Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide.
A0758-1 14-3-3 protein theta - Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide.
Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog
Rabbit, Mouse, Goat, Sheep
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C)
Background of 14-3-3 protein theta antibody
At least seven isoforms comprise the highly conserved 14-3-3 family of homo-and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. These multifunctional proteins bind and modulate the function of a wide array of cellular proteins. They exist mainly as dimers with a monomeric molecular mass of approximately 30 kDa and have an acidic isoelectric point of 4.5-5. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with bad, prevents apoptosis.3 14-3-3 plays a dual role in Raf-1 activation. It helps to keep Raf-1 in an inactive state by binding to phophoserine-259 and the cysteine-rich domain (DRD). Interactions of 14-3-3 at the phosphoserine-621 may be required for Raf-1 activation and may function as an essential cofactor for Raf-1 kinase activity.3 Other proteins that have been shown to bind 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, middle-T antigen, KSR, IGF-1 receptor, etc.3 Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases. The theta or tau isoform was initially isolated from T cells.1 It
Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M. Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle. Diabetes. 2007 Jun;56(6):1608-14. Epub 2007 Mar 16. PMID: 17369524
Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide.
Western Blot: 14-3-3 theta Antibody [NBP1-71900]- Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-14-3-3-theta antibody used for IP at 6 mcg/mg lysate. 14-3-3-theta was also immunoprecipitated by rabbit anti-14-3-3-theta antibody NBP1-71901, which recognizes a downstream epitope.
Western Blot: 14-3-3 theta Antibody [NBP1-71901]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-14-3-3-theta antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. 14-3-3-theta was also immunoprecipitated by rabbit anti-14-3-3-theta antibody NBP1-71900, which recognizes an upstream epitope.
Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.