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14-3-3 protein theta antibody

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Principal name

14-3-3 protein theta antibody

Alternative names for 14-3-3 protein theta antibody

14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1, FocusOn116

Selected pictures

14-3-3 protein theta antibody - _1
_1 - Western blot (WB) analyzes of 14-3-3 θ antibody in extracts from HUVEC cells.
14-3-3 protein theta antibody - AM05264PU-N
AM05264PU-N - Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3), Jurkat (Lane 4) and normal human fibroblasts (Lane 5).

SwissProt ID

P27348 (Human)

Gene ID

10971 (YWHAQ)

Ncbi ID

NP_006817.1, 9606, NP_006817

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Rb (Rabbit), Sh (Sheep), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine)

Available hosts

Mouse, Sheep, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of 14-3-3 protein theta antibody

At least seven isoforms comprise the highly conserved 14-3-3 family of homo-and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. These multifunctional proteins bind and modulate the function of a wide array of cellular proteins. They exist mainly as dimers with a monomeric molecular mass of approximately 30 kDa and have an acidic isoelectric point of 4.5-5. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with bad, prevents apoptosis.3 14-3-3 plays a dual role in Raf-1 activation. It helps to keep Raf-1 in an inactive state by binding to phophoserine-259 and the cysteine-rich domain (DRD). Interactions of 14-3-3 at the phosphoserine-621 may be required for Raf-1 activation and may function as an essential cofactor for Raf-1 kinase activity.3 Other proteins that have been shown to bind 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, middle-T antigen, KSR, IGF-1 receptor, etc.3 Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases. The theta or tau isoform was initially isolated from T cells.1 It

General readings

1. Nielsen, P.J., Primary structure of a human protein kinase regulator protein. Biochim. Biophys. Acta, 1088,425-428 (1991).
2. Xiao, B., et al., Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways. Nature, 376, 188-191 (1995).
3. Fu, H., et al., 14-3-3 proteins: structure, function, and regulation. Annu Rev Pharmacol Toxicol., 40, 617-47 (2000).
4. Skoulakis, E.M., and Davis, R.L., 14-3-3 proteins in neuronal development and function. Mol. Neurobiol., 16, 269-84. (1998).
5. Baldin, V., 14-3-3 proteins and growth control. Prog. Cell Cycle Res., 4, 49-60. (2000).

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26 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
14503-1-AP

14-3-3 protein theta antibody

Immunofluorescent analysis of HepG2 cells, using 14503-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Rabbit Aff - Purified Hu, Ms E, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
+1 additional image
 
AP05571SU-N

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Serum Bov, Chk, Hu, Ms, Rb, Rt, Sh E, WB
0.1 ml / €430.00
  IgG   Acris Antibodies GmbH
 
AP06768PU-N

14-3-3 protein theta antibody

Western blot (WB) analyzes of 14-3-3 θ antibody in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
AP20329PU-N

14-3-3 protein theta antibody

Western blot analysis of 14-3-3 θ/τ antibody (AP20329PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Ms, Rt ICC/IF, P, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
NB100-92414

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  IgG   Novus Biologicals Inc.
 
NB100-92417

14-3-3 protein theta antibody

Western Blot: 14-3-3 Theta Antibody [NB100-92417] - (WB) analyzes of 14-3-3 è (Q67) antibody in extracts from HUVEC cells.. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  IgG   Novus Biologicals Inc.
+1 additional image
 
NBP1-19488

14-3-3 protein theta antibody

Western Blot: 14-3-3 theta/tau Antibody [NBP1-19488] - extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €370.00
    Novus Biologicals Inc.
 
NBP1-31537

14-3-3 protein theta antibody

Western Blot: 14-3-3 Tau Antibody [NBP1-31537] - Western Blot analysis of NBP1-31537 on: A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE gel, diluted at 1:1000. Rabbit Hu P, WB
0.1 ml / €390.00
  IgG   Novus Biologicals Inc.
 
NBP1-52856

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Purified Hu E
0.2 ml / €370.00
    Novus Biologicals Inc.
 
NBP1-61494-0.1

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €340.00
  IgG   Novus Biologicals Inc.
 
NBP1-61494-50

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €290.00
  IgG   Novus Biologicals Inc.
 
NBP1-68208-0.1

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €340.00
  IgG   Novus Biologicals Inc.
 
NBP1-68208-50

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  IgG   Novus Biologicals Inc.
 
NBP1-71900

14-3-3 protein theta antibody

Western Blot: 14-3-3 theta Antibody [NBP1-71900]- Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-14-3-3-theta antibody used for IP at 6 mcg/mg lysate. 14-3-3-theta was also immunoprecipitated by rabbit anti-14-3-3-theta antibody NBP1-71901, which recognizes a downstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €370.00
    Novus Biologicals Inc.
 
NBP1-71901

14-3-3 protein theta antibody

Western Blot: 14-3-3 theta Antibody [NBP1-71901]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-14-3-3-theta antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. 14-3-3-theta was also immunoprecipitated by rabbit anti-14-3-3-theta antibody NBP1-71900, which recognizes an upstream epitope. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €370.00
    Novus Biologicals Inc.
 
NBP1-90337

14-3-3 protein theta antibody

Western Blot: 14-3-3 theta Antibody [NBP1-90337] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil Rabbit Aff - Purified Hu C, ICC/IF, P, WB
0.1 ml / €360.00
  IgG   Novus Biologicals Inc.
+2 additional images
 
GTX102199

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Aff - Purified Hu WB
0.1 ml / €300.00
    GeneTex Inc.
 
GTX112026

14-3-3 protein theta antibody

14-3-3 protein theta Rabbit Aff - Purified Hu WB
0.1 ml / €300.00
    GeneTex Inc.
 
PAB14303

14-3-3 protein theta antibody

Western blot analysis of YWHAQ expression in HeLa ( Lane 1 ) and Jurkat ( Lane 2 ) whole cell lysates. Using YWHAQ polyclonal antibody ( Cat # PAB14303 ). Rabbit Hu, Ms, Rt C, IP, WB
0.2 mg / €330.00
    Abnova Taiwan Corp.
 
A0758-1

14-3-3 protein theta pSer232 antibody

Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €195.00
    Assay Biotech
+2 additional images
 

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