ABCC8 antibody

Principal name

ABCC8 antibody

Alternative names for ABCC8 antibody

HRINS, SUR, SUR1, Sulfonylurea receptor 1, ATP-binding cassette transporter sub-family C member 8

Selected pictures

ABCC8 antibody - H00006833-M01
H00006833-M01 - Detection limit for recombinant GST tagged ABCC8 is approximately 0.03ng/ml as a capture antibody.
ABCC8 antibody - NBP1-00138 ABCC8
NBP1-00138 ABCC8 - NBP1-00138 (0.5ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SwissProt ID

Q09428 (Human), Q09429 (Rat)

Gene ID

6833 (ABCC8), 25559 (Abcc8)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Hst (Hamster)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ABCC8 antibody

The relation ship between glucose metabolism and insulin secretion from the pancreatic beta cells is linked with ATP-sensitive potassium channels (KATP). IN response to glucose metabolism, the ATP-sensitive potassium channels are inactivated due to an increase in ATP/ADP ratio, resulting in membrane depolarization and calcium influx through voltage dependent L-type Ca+2 channels. The KATPs are composed 4 units of inwardly rectifying K channel subunit (Kir6.2) and 4 copies of regulatory subunit SUR1 in an octameric complex. SUR 1 is a member of ATP binding cassette super family. SUR receptor confers the sensitivity of Kir6.2 to ATP/ADP sensitivity and to pharmacological agents such as sulfonylurea and diazoxide that close or open the KATP channels. The persistent heyperinsulinimic hypoglycemia in infancy (PHHI) is familial disorder due to defect in negative feed back in response to low glucose levels. SUR1 was mapped on chromosome 11p14-15.1, the same location where the gene for PHHI is located (1). It has been shown that the expression of Kir6.2 and SUR1 are regulated by glucose levels and the actives of glucogon-like peptide receptor1 (2). Abnormal insulin secretion in PHHI appears to be caused by mutations in the SUR gene.

SUR1 is a 1581 amino acid protein with an ATP binding domain with two nucleotide-binding folds (NBFs) and binding sites for sulfonylureas, like glibenclamide, and for channel openers. SUR contains three hydrophobic domains, TM(0), TM(1), and TM(2), with nucleotide binding folds following TM(1) and TM(2). The protein is glycosylated at position 10 on Asn. The SUR1 gene is expressed in at least 4 multiple splice variants ere are multiple splice variants, these splice variants originate from the splicing of the coding region of NFB and lack of axon 17 (SUR1Delta17), axon 19 (SUR1Delta 19) and both (SUR1Delta 17/19) and the fourth is a COOH terminal fragment formed by Exxon 31-39 containing the last 2 TMD and the COOH terminal (SUR1C). The various splice variants of SUR1 are expressed in various tissues with strong expression of SUR1C in cardiomyocytes (3). Only SUR1 and SUR1Delta 17 exhibit high affinity binding sites for sulfonylurea and other pharmacological agents. Several point mutations in the NFB region of SUR1 are also described in NIDDM. A total of five amino acid substitutions and 17 silent mutations were noted by examining all 39 axons of this gene in NIDDM patients form Japan. Two rare novel mutations, D811N in axon 20 and R835C in axon 21, were identified in the first nucleotide-binding fold (NBF), a functionally important region of SUR1, in one patient each, both heterozygotes.

General readings

de Wet H, Rees MG, Shimomura K, Aittoniemi J, Patch AM, Flanagan SE, Ellard S, Hattersley AT, Sansom MS, Ashcroft FM.
Increased ATPase activity produced by mutations at arginine-1380 in nucleotidebinding domain 2 of ABCC8 causes neonatal diabetes.
Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18988-92.
PMID: 18025464

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Antibodies

Catalog No. Host Clone/Iso. Pres. React. Applications  
AM60008PU-N

ABCC8 (1548-1582, Cytopl. Dom.) antibody

ABCC8 Mouse S289-16 Purified Hst, Ms, Rt ICC/IF, P, WB
0.1 mg / €360.00
  IgG1   Acris Antibodies GmbH
 
AP10332CP-N

ABCC8 Control Peptide

ABCC8
0.25 mg / €150.00
    Acris Antibodies GmbH
 
AP10332PU-N

ABCC8 antibody

ABCC8 Rabbit Aff - Purified Hu Dot, E, WB
0.1 mg / €350.00
  IgG   Acris Antibodies GmbH
 
AP17054PU-N

ABCC8 (C-term) antibody

Antibody (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €270.00
    Acris Antibodies GmbH
 
19600002

ABCC8 antibody

Immunohistochemistry: SUR1 Antibody [19600002] - Most normal tissues exhibited moderate to strong cytoplasmic expression. Membranous staining pattern was seen in the intestinal tissues. Strong cytoplasmic immunoreactivity was observed in salivary gland, exocrine pancreas, skeletal muscle and non-follicle cells of lymph node. Purkinje cells and adrenal cortical cells demonstrated strong nuclear positivity. Malignant tissues generally displayed weak to moderate cytoplasmic expression. Strong membranous staining pattern was observed in colo-rectal cancers. Nuclear staining was mainly seen in non-Hodgkin's lymphomas, urothelial cancers and malignant gliomas.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit Hu E, P
50 µg / €430.00
    Novus Biologicals Inc.
 
NBP1-00138

ABCC8 antibody

NBP1-00138 (0.5ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €370.00
  IgG   Novus Biologicals Inc.
 
NBP1-59778

ABCC8 antibody

Western Blot: SUR1 Antibody [NBP1-59778] - This Anti-ABCC8 antibody was used in Western Blot of 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €390.00
    Novus Biologicals Inc.
 
GTX88616

ABCC8 C-term antibody

ABCC8 Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €330.00
  IgG   GeneTex Inc.
 
H00006833-A01

ABCC8 antibody

ABCC8 Mouse Serum Hu E, WB
50 µl / €260.00
    Abnova Taiwan Corp.
 
H00006833-B01P

ABCC8 antibody

ABCC8 Mouse Hu WB
0.5 mg / €1,420.00
    Abnova Taiwan Corp.
 
H00006833-M01

ABCC8 antibody

Detection limit for recombinant GST tagged ABCC8 is approximately 0.03ng/ml as a capture antibody. Mouse 3G5 Purified Hu E, WB
0.1 mg / €340.00
  IgG2a   Abnova Taiwan Corp.
 
H00006833-M01A

ABCC8 antibody

ABCC8 Mouse 3G5 Ascites Hu E, WB
0.2 ml / €340.00
  IgG2a   Abnova Taiwan Corp.
 
H00006833-M02

ABCC8 antibody

ABCC8 Mouse 3E1 Purified Hu E, WB
0.1 mg / €340.00
  IgG1   Abnova Taiwan Corp.
 
ARP43622_P050

ABCC8 antibody

ABCC8 Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €330.00
    AVIVA Systems Biology
 

Proteins

Catalog No. Species Pres. Purity Source  
H00006833-Q01-10

ABCC8

ABCC8 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
 
H00006833-Q01-25

ABCC8

ABCC8 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
 

16 Item(s)

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