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AKR1D1 / SRD5B1 antibody

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Principal name

AKR1D1 / SRD5B1 antibody

Alternative names for AKR1D1 / SRD5B1 antibody

3-oxo-5-beta-steroid 4-dehydrogenase, Delta(4)-3-ketosteroid 5-beta-reductase, Delta(4)-3-oxosteroid 5-beta-reductase, Aldo-keto reductase family 1 member D1

Selected pictures

AKR1D1 / SRD5B1 antibody - H00006718-M03
H00006718-M03 - Western Blot analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2.Lane 1: AKR1D1 transfected lysate(37.4 KDa).Lane 2: Non-transfected lysate.
AKR1D1 / SRD5B1 antibody - H00006718-M03
H00006718-M03 - Western blot analysis of AKR1D1 over-expressed 293 cell line, cotransfected with AKR1D1 Validated Chimera RNAi ( Cat # H00006718-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AKR1D1 monoclonal antibody (M03), clone 1C2 (Cat # H00006718-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

SwissProt ID

P31210 (Rat), P51857 (Human), Q8VCX1 (Mouse)

Gene ID

6718 (AKR1D1)

Ncbi ID

9606, NP_005980, NM_005989, NP_005980.1

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of AKR1D1 / SRD5B1 antibody

Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

1. Steen, N.E., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry (2010)
2. Drury, J.E., et al. J. Biol. Chem. 285(32):24529-24537(2010)
3. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
4. Ueki, I., et al. J. Gastroenterol. Hepatol. 24(5):776-785(2009)
5. Panagopoulos, I., et al. Oncol. Rep. 21(3):615-624(2009)

Items 1 to 20 of 22 total

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22 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AP50135PU-N

AKR1D1 / SRD5B1 (Center) antibody

AKR1D1 Antibody (Center) (Cat. #AP50135PU-N) western blot analysis in 293 cell line lysates (35 µg/lane).This demonstrates the AKR1D1 antibody detected the AKR1D1 protein (arrow). Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
 
NBP1-74082

AKR1D1 / SRD5B1 antibody

AKR1D1 / SRD5B1 Rabbit Aff - Purified Hu WB
50 µg / €390.00
    Novus Biologicals Inc.
 
GTX109328

AKR1D1 / SRD5B1 antibody

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GTX109328 diluted at 1:1000 Rabbit N1C3 Purified Hu
0.1 ml / €300.00
  IgG   GeneTex Inc.
+1 additional image
 
GTX117738

AKR1D1 / SRD5B1 antibody

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GTX117738 diluted at 1:1000 Rabbit Aff - Purified Hu WB
0.1 ml / €300.00
    GeneTex Inc.
+1 additional image
 
H00006718-B01P

AKR1D1 / SRD5B1 antibody

Western Blot analysis of AKR1D1 expression in transfected 293T cell line (H00006718-T01) by AKR1D1 MaxPab polyclonal antibody. Lane1:AKR1D1 transfected lysate(35.86 KDa). Lane2:Non-transfected lysate. Mouse Purified E(detection), WB
50 µg / €340.00
    Abnova Taiwan Corp.
+1 additional image
 
H00006718-D01P

AKR1D1 / SRD5B1 antibody

Western Blot analysis of AKR1D1 expression in transfected 293T cell line (H00006718-T01) by AKR1D1 MaxPab polyclonal antibody. Lane 1: AKR1D1 transfected lysate(37.40 KDa). Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €360.00
    Abnova Taiwan Corp.
 
H00006718-M01

AKR1D1 / SRD5B1 antibody

AKR1D1 / SRD5B1 Mouse 1A6 Purified Hu E
0.1 mg / €340.00
  IgG2b   Abnova Taiwan Corp.
 
H00006718-M03

AKR1D1 / SRD5B1 antibody

Western Blot analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2. Lane 1: AKR1D1 transfected lysate(37.4 KDa). Lane 2: Non-transfected lysate. Mouse 1C2 Purified Hu E, WB
0.1 mg / €340.00
  IgG2b   Abnova Taiwan Corp.
+2 additional images
 
ARP60804_P050

AKR1D1 / SRD5B1 antibody

AKR1D1 / SRD5B1 Rabbit Aff - Purified Hu WB
50 µg / €330.00
    AVIVA Systems Biology
 

Proteins

Catalog No. Species Pres. Purity   Source  
AR09893PU-L

AKR1D1 / SRD5B1 (1-326, His-tag)

Recombinant human AKR1D1, 1-326 aa, His-tagged Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH
+1 additional image
 
AR09893PU-N

AKR1D1 / SRD5B1 (1-326, His-tag)

Recombinant human AKR1D1, 1-326 aa, His-tagged Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH
+1 additional image
 
NBP1-51026-0.1

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified
0.1 mg / €340.00
  Novus Biologicals Inc.
 
NBP1-51026-0.5

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified
0.5 mg / €740.00
  Novus Biologicals Inc.
 
H00006718-P01-10

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
 
H00006718-P01-25

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.
 
H00006718-Q01-10

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
 
H00006718-Q01-25

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
 

Lysates

Catalog No. Name  
H00006718-T01

AKR1D1 293T Cell Transient Overexpression Lysate(Denatured)

AKR1D1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €240.00
  Abnova Taiwan Corp.
 
NBL1-07438

AKR1D1 Lysate

Western Blot: AKR1D1 Lysate [NBL1-07438] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for AKR1D1  
0.1 mg / €370.00
  Novus Biologicals Inc.
 

Antibody Pairs

Catalog No. Name  
H00006718-PW1

AKR1D1 (Human) IP-WB Antibody Pair

AKR1D1 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.
 

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Product Type
  1. Antibodies (9)
  2. Antibody Pairs (3)
  3. Lysates (2)
  4. Proteins (8)
Antibody type
  1. Antibody (Ab) (9)
Clonality
  1. Polyclonal (7)
  2. Monoclonal (3)
Target
  1. AKR1D1 / SRD5B1 (17)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (10)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Enzyme Immunoassay (E) (3)
  3. ELISA (detection) (E(detection)) (2)
Presentation
  1. Purified (11)
  2. Aff - Purified (4)
Host
  1. Rabbit (6)
  2. Mouse (3)
Images
  1. 1 image (9)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. SDS-PAGE (2)
  3. ELISA (capture) (E(capture)) (1)
  4. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (8)
Source
  1. E. coli (2)
  2. in vitro transl. (2)
Manufacturer
  1. Acris Antibodies GmbH (3)
  2. Abnova Taiwan Corp. (12)
  3. Novus Biologicals Inc. (4)
  4. GeneTex Inc. (2)
  5. AVIVA Systems Biology (1)
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