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APRT antibody

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Principal name

APRT antibody

Alternative names for APRT antibody

Adenine phosphoribosyltransferase

Selected pictures

APRT antibody - H00000353-B01P
H00000353-B01P - Western Blot analysis of APRT expression in transfected 293T cell line (H00000353-T01) by APRT MaxPab polyclonal antibody.Lane 1: APRT transfected lysate(19.8 KDa).Lane 2: Non-transfected lysate.
APRT antibody - H00000353-B01P
H00000353-B01P - APRT MaxPab polyclonal antibody. Western Blot analysis of APRT expression in human liver.

SwissProt ID

P07741 (Human)

Gene ID

353 (APRT)

Ncbi ID

9606, NP_000476, NP_000476.1

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of APRT antibody

Adenine phosphoribosyltransferase (APRT) belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

General readings

Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008) Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)

20 Item(s)

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
21405-1-AP

APRT antibody

A549 cell were subjected to SDS PAGE followed by western blot with 21405-1-AP(APRT Antibody) at dilution of 1:800 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
 
AP17121CP-N

APRT Control Peptide

APRT
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP17121PU-N

APRT (C-term) antibody

Western blot analysis of APRT (arrow) using rabbit polyclonal APRT Antibody (C-term) (AP17121PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the APRT gene (Lane 2) (Origene Technologies). Rabbit Hu E, WB
0.1 mg / €330.00
    Acris Antibodies GmbH
 
AP17122CP-N

APRT Control Peptide

APRT
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP17122PU-N

APRT (N-term) antibody

(LEFT) Western blot analysis of APRT Antibody (N-term) (AP17122PU-N) in A2058,MCF-7 cell line lysates (35 µg/lane). APRT (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of APRT (arrow) using rabbit polyclonal APRT Antibody (N-term) (AP17122PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the APRT gene (Lane 2) (Origene Technologies). Rabbit Hu E, P, WB
0.1 mg / €330.00
    Acris Antibodies GmbH
+1 additional image
 
NBP1-31332

APRT antibody

Western Blot: APRT Antibody [NBP1-31332] - Western blot analysis of NBP1-31332 on A431, with 12% SDS PAGE, diluted at 1:1000. Rabbit Hu ICC/IF, P, WB
0.1 mg / €390.00
  IgG   Novus Biologicals Inc.
+1 additional image
 
NBP1-55300

APRT antibody

Western Blot: APRT Antibody [NBP1-55300] - This Anti-APRT antibody was used in Western Blot of 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €390.00
    Novus Biologicals Inc.
 
NBP1-89519

APRT antibody

Immunohistochemistry-Paraffin: APRT Antibody [NBP1-89519] - Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €360.00
  IgG   Novus Biologicals Inc.
+2 additional images
 
GTX109594

APRT antibody

APRT Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €300.00
    GeneTex Inc.
 
H00000353-B01P

APRT antibody

Western Blot analysis of APRT expression in transfected 293T cell line (H00000353-T01) by APRT MaxPab polyclonal antibody. Lane 1: APRT transfected lysate(19.8 KDa). Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €340.00
    Abnova Taiwan Corp.
+1 additional image
 
H00000353-D01

APRT antibody

APRT Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €360.00
    Abnova Taiwan Corp.
 
H00000353-D01P

APRT antibody

Western Blot analysis of APRT expression in transfected 293T cell line (H00000353-T01) by APRT MaxPab polyclonal antibody. Lane 1: APRT transfected lysate(19.60 KDa). Lane 2: Non-transfected lysate. Rabbit Purified E(detection), WB
0.1 mg / €360.00
    Abnova Taiwan Corp.
 
ARP58422_P050

APRT antibody

APRT Rabbit Aff - Purified Bov, Can, Hu, Rt WB
50 µg / €330.00
    AVIVA Systems Biology
 

Proteins

Catalog No. Species Pres. Purity   Source  
AR09382PU-L

APRT (1-180)

Recombinant human APRT, 1-180 aa: 15% SDS-PAGE (3 µg) Human Purified > 90 % E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH
+1 additional image
 
AR09382PU-N

APRT (1-180)

Recombinant human APRT, 1-180 aa: 15% SDS-PAGE (3 µg) Human Purified > 90 % E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH
+1 additional image
 
NBP1-40402

APRT

SDS-Page: APRT Protein [NBP1-40402] - APRT, 19.6 kDa (180aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Liquid
0.1 mg / €310.00
  Novus Biologicals Inc.
 
H00000353-P01-10

APRT

APRT Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
 
H00000353-P01-25

APRT

APRT Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.
 

Lysates

Catalog No. Name  
H00000353-T01

APRT 293T Cell Transient Overexpression Lysate(Denatured)

APRT 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €240.00
  Abnova Taiwan Corp.
 

Antibody Pairs

Catalog No. Name  
H00000353-PW1

APRT (Human) IP-WB Antibody Pair

APRT (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.
 

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Product Type
  1. Antibodies (13)
  2. Antibody Pairs (1)
  3. Lysates (1)
  4. Proteins (5)
Antibody type
  1. Antibody (Ab) (11)
  2. Control peptide (Ab/CP) (2)
Clonality
  1. Polyclonal (13)
Target
  1. APRT (18)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (11)
  2. Rat (Rt) (2)
  3. Bovine (Bov) (1)
  4. Canine (Can) (1)
  5. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (11)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (3)
  4. ELISA (detection) (E(detection)) (3)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
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  2. 3+ images (1)
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  1. Western blot / Immunoblot (WB) (9)
  2. SDS-PAGE (3)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  4. Paraffin Sections (P) (2)
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  1. Human (4)
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  1. E. coli (2)
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  1. Acris Antibodies GmbH (6)
  2. Abnova Taiwan Corp. (7)
  3. Novus Biologicals Inc. (4)
  4. AVIVA Systems Biology (1)
  5. GeneTex Inc. (1)
  6. ProteinTech Group, Inc. (1)
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