Alternative names for Bax antibody
Apoptosis regulator BAX, BCL2L4, Bcl2-L-4, Bcl-2-like protein 4, BCL2-associated X protein
Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Radial Immunodiffusion (RID), Flow Cytometry (F)
Background of Bax antibody
The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down’s syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy.
1. Garg P, Nemec KN, Khaled AR, Tatulian SA. Transmembrane pore formation by the carboxyl terminus of Bax protein. Biochim Biophys Acta. 2013 Feb;1828(2):732-42. doi: 10.1016/j.bbamem.2012.08.006. Epub 2012 Aug 18. PubMed PMID: 22906710.
2. Bose P, Klimowicz AC, Kornaga E, Petrillo SK, Matthews TW, Chandarana S, et al. Bax expression measured by AQUAnalysis is an independent prognostic marker in oral squamous cell carcinoma. BMC Cancer. 2012 Aug 1;12:332. doi: 10.1186/1471-2407-12-332. PubMed PMID: 22852863.
3. Ma J, Edlich F, Bermejo GA, Norris KL, Youle RJ, Tjandra N. Structural mechanism of Bax inhibition by cytomegalovirus protein vMIA. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20901-6. doi: 10.1073/pnas.1217094110. Epub 2012 Dec 3. PubMed PMID: 23213219.
4. Sun Y, Zhao X, Yao Y, Qi X, Yuan Y, Hu Y. Connexin 43 interacts with Bax to regulate apoptosis of pancreatic cancer through a gap junction-independent pathway. Int J Oncol. 2012 Sep;41(3):941-8. doi: 10.3892/ijo.2012.1524. Epub 2012 Jun 20. PubMed PMID: 22736223.
5. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
6. Cartron PF, et al. 2004. FEBS Lett. 578:41.
7. Pucci S, et al. 2009. Cell Cycle 8:473.
8. LeBlanc H, et al. 2002. Nat. Med. 8:274.
9. Marzo I, et al. 1998. Science 281:2027.
10. Miyashita T et al. 1995. Cell 80:293.
11. Oltvai ZN, et al. 1993. Cell 74:609.
Choose your store!
Please go to the corresponding page of the website for your country/region to buy our products
Choose this store if you are located in…
- America (e.g. United-States, Canada, Mexico, Brazil, …)
- Asia (e.g. Japan, Hong-Kong, China, South Korea, Taiwan, …)
- Australia (e.g. Australia, New Zealand, …)
Sorry for the inconvenience, maybe you should bookmark the right store for your next visit.