Bax antibody

Principal name

Bax antibody

Alternative names for Bax antibody

Apoptosis regulator BAX, BCL2L4, Bcl2-L-4, Bcl-2-like protein 4, BCL2-associated X protein

Selected pictures

Bax antibody - AM00183PU-N
AM00183PU-N - Western blot analysis of Bax expression in HeLa cell lysate.
Bax antibody - AP00015PU-N
AP00015PU-N - Western blot analysis of Bax expression in rat kidney tissue lysate.

SwissProt ID

O02703 (Bovin), Q07812 (Human), Q07813 (Mouse), Q63690 (Rat)

Gene ID

581 (BAX), 24887 (Bax), 12028, 5644 (PRSS1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Fe (Feline), Mky (Monkey), Ge (Gerbil), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Bax antibody

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down’s syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy.

General readings

Bae J et al. Underphosphorylated BAD interacts with diverse antiapoptotic Bcl-2 family proteins to regulate apoptosis. Apoptosis 6:319-30 (2001). PubMed PMID: 11483855
Fridman JS et al. Cytochrome c depletion upon expression of Bcl-XS. J Biol Chem 276:4205-10 (2001). PubMed PMID: 11044452
Coldwell MJ et al. The p36 isoform of BAG-1 is translated by internal ribosome entry following heat shock. Oncogene 20:4095-100 (2001). PubMed PMID: 11494137
Wei MC et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292:727-30 (2001). PubMed PMID: 11326099
Lindenboim L et al. Bcl-xS and Bax induce different apoptotic pathways in PC12 cells. Oncogene 19:1783-93 (2000). PubMed PMID: 10777212
Huang Z Bcl-2 family proteins as targets for anticancer drug design. Oncogene 19:6627-31 (2000). PubMed PMID: 11426648
Eversole-Cire P et al. Synergistic effect of Bcl-2 and BAG-1 on the prevention of photoreceptor cell death. Invest Ophthalmol Vis Sci 41:1953-61 (2000). PubMed PMID: 10845622
Reed JC Double identity for proteins of the Bcl-2 family. Nature 387:773-6 (1997). PubMed PMID: 9194558
Yang E et al. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Cell 80:285-91 (1995). PubMed PMID: 7834748

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