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CD220 / INSR antibody

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Principal name

CD220 / INSR antibody

Alternative names for CD220 / INSR antibody

Insulin Receptor, FocusOn060

Selected pictures

CD220 / INSR antibody - AM00076PU-N
AM00076PU-N - Phosphospecificity Whole cell extracts of control (co) or Insulin, IGF1, Pervanadate stimulated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with Monoclonal InsR antibody 21G12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: untreated Lane 2: Insulin-treated Lane 3: IGF1-treatedLane 4: Pervanadate-treated
CD220 / INSR antibody - AM00077PU-N
AM00077PU-N - Detection of endogenous InsR Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-9H4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D

SwissProt ID

P06213 (Human), P15127 (Rat), P15208 (Mouse), Q9PVZ4 (Xenla)

Gene ID

3643 (INSR), 11255 (HRH3), 16337 (Insr), 3736432 (IPR000438)

Ncbi ID

NP_000199.2, NP_000199, 9606, NM_010568, NP_034698, NP_034698.2

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Por (Porcine), Sh (Sheep), Hst (Hamster), Rb (Rabbit), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Neutralisation (NEUT), Functional assay (FN), ELISA (capture) (E(capture)), Paraffin Sections (P), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD220 / INSR antibody

The insulin receptor is a heterotetrameric membrane glycoprotein with tyrosine-protein kinase activity, consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit possesses a single transmembrane domain, whereas the alpha subunit is completely extracellular. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Binding of insulin to the insulin receptor stimulates its association with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K) which leads to glucose uptake. Two transcript variants encoding different isoforms have been found for this gene produced by alternative splicing. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

General readings

1. Forsayeth, J.R., et al. Effect of monoclonal antibodies on human insulin receptor autophosphorylation, negative cooperativity, and down-regulation. JBC. 262(9): 4134-4140, 1987. (Immunoprecipitation, Compet. assay, human)
2. Frasca, F., et al. Insulin receptor isoform A, a newly recognized, high-affinity insulin-like growth factor II receptor in fetal and cancer cells. Molec. & Cell. Biol. 19(5):3278-3288, 1999. (ELISA, Immunoprecipitation, Western Blot, Human)
3. Federici, M., et al. Increased expression of insulin/insulin-like growth factor-I hybrid receptors in skeletal muscle of noninsulin-dependent diabetes mellitus subjects. J. Clin. Invest. 98(12):2887-2893, 1996. (Western Blot, Human)
4. Li, M., et al. Small molcule insulin receptor activators potentiate insulin action in insulin-resistant cells. Diabetes. 50:2323-2328, 2001. (ELISA, Western Blot, Rat)

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81 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
20433-1-AP

CD220 / INSR antibody

293 cell were subjected to SDS PAGE followed by western blot with 20433-1-AP(INSR Antibody) at dilution of 1:1000 Rabbit Aff - Purified Hu E, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
 
AM00076PU-N

CD220 / INSR pTyr1322 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (co) or Insulin, IGF1, Pervanadate stimulated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with Monoclonal InsR antibody 21G12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: untreated Lane 2: Insulin-treated Lane 3: IGF1-treated Lane 4: Pervanadate-treated Mouse 21G12 Purified Can, Hu, Ms E, WB
0.1 mg / €390.00
  IgG1   Acris Antibodies GmbH
 
AM00077PU-N

CD220 / INSR (beta chain) (incl. pos. control) antibody

Detection of endogenous InsR Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-9H4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D Mouse 9H4 Purified Can, Hu, Ms, Rt C, E, WB
0.1 mg / €390.00
  IgG1   Acris Antibodies GmbH
 
AM00078PU-N

CD220 / INSR (beta chain) (incl. pos. control) antibody

Detection of endogenous InsR Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-11B6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A Mouse 11B6 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €390.00
  IgG1   Acris Antibodies GmbH
 
AM00362PU-N

CD220 / INSR (Beta subunit) antibody

Formalin-fixed, paraffin-embedded human placenta stained with Insulin Receptor antibody (AM00362PU-N) using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of trophoblasts. Mouse CT-3 Purified Hu, Mky, Ms, Rt E(capture), P, WB
0.1 mg / €480.00
  IgG1   Acris Antibodies GmbH
+1 additional image
 
AM21037PU-N

CD220 / INSR (alpha chain) antibody

CD220 / INSR Mouse 83-14 Azide Free Hu FN, IP
0.1 mg / €370.00
  IgG2a   Acris Antibodies GmbH
 
AM21038PU-N

CD220 / INSR antibody

CD220 / INSR Mouse 83-7 Azide Free Hu IP
0.1 mg / €370.00
  IgG1   Acris Antibodies GmbH
 
AM21039PU-N

CD220 / INSR (alpha chain) antibody

CD220 / INSR Mouse 47-9 Azide Free Hu FN
0.1 mg / €370.00
  IgG1   Acris Antibodies GmbH
 
AM21040PU-N

CD220 / INSR (beta chain) antibody

CD220 / INSR Mouse 18-44 Azide Free Hu FN, IP, WB
0.1 mg / €370.00
  IgG2b   Acris Antibodies GmbH
 
AP01347PU-N

CD220 / INSR antibody

Western blot (WB) analysis of IR antibody in extracts from LOVO cells. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
AP07100PU-N

CD220 / INSR (beta chain) antibody

AP07100PU-N CD220/INSR antibody staining of Formalin-Fixed Paraffin-Embedded Human Pancreas, Islet of Langerhans at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Hu P
50 µg / €380.00
    Acris Antibodies GmbH
 
AP07101PU-N

CD220 / INSR (beta chain) antibody

AP07101PU-N CD220/INSR antibody staining of Formalin-Fixed Paraffin-Embedded Human Pancreas, Islet of Langerhans at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Hu P
50 µg / €380.00
    Acris Antibodies GmbH
 
AP12631CP-N

CD220 / INSR control peptide

CD220 / INSR
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP12631PU-N

CD220 / INSR pTyr1361 antibody

Dot blot analysis of anti-INSR-pY1361 Pab (Cat#AP12631PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Aff - Purified Hu E, P
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
+1 additional image
 
AP12966CP-N

CD220 / INSR Control Peptide

CD220 / INSR
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP12966PU-N

CD220 / INSR pTyr1185 antibody

Dot blot analysis of anti-Phospho-INSR pTyr1185 Antibody (Cat.#AP12966PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Aff - Purified Hu E
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
 
AP14370CP-N

CD220 / INSR Control Peptide

CD220 / INSR
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP14370PU-N

CD220 / INSR (N-term) antibody

Western blot analysis of anti-INSR Pab in SKBR-3 cell lysate. INSR (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Purified Hu E, P, WB
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
+1 additional image
 
AP14371CP-N

CD220 / INSR Control Peptide

CD220 / INSR
0.1 mg / €120.00
    Acris Antibodies GmbH
 
AP14371PU-N

CD220 / INSR antibody

Western blot analysis of anti-INSR Antibody (Y1185) in mouse lung tissue lysates (35ug/lane). INSR(arrow) was detected using the purified Pab. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
+1 additional image
 

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  2. Monoclonal (26)
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Reactivity
  1. Human (Hu) (69)
  2. Mouse (Ms) (29)
  3. Rat (Rt) (26)
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  5. Zebrafish (Ze) (3)
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  10. African clawed frog (1)
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  1. Western blot / Immunoblot (WB) (46)
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  6. Neutralisation (NEUT) (5)
  7. Frozen sections (C) (4)
  8. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  9. Dot blot (Dot) (3)
  10. ELISA (capture) (E(capture)) (1)
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  1. 1 image (41)
Images: Applications
  1. Western blot / Immunoblot (WB) (28)
  2. Paraffin Sections (P) (16)
  3. Dot blot (Dot) (5)
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  5. Enzyme Immunoassay (E) (1)
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  1. in vitro transl. (2)
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  6. QED Bioscience Inc. (4)
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