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DNAJB1 / HSP40 antibody

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Principal name

DNAJB1 / HSP40 antibody

Alternative names for DNAJB1 / HSP40 antibody

Heat shock 40 kDa protein 1, HSP-40, DnaJ protein homolog 1, HDJ-1, DNAJ1, HDJ1, HSPF1, Review 003, DnaJ homolog subfamily B member 1

Selected pictures

DNAJB1 / HSP40 antibody - 13174-1-AP DNAJB1 / HSP40
13174-1-AP DNAJB1 / HSP40 - Immunofluorescent analysis of Hela cells, using 13174-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).
DNAJB1 / HSP40 antibody - 13174-1-AP DNAJB1 / HSP40
13174-1-AP DNAJB1 / HSP40 - Immunohistochemistry of paraffin-embedded Lymphoma using 13174-1-AP(DNAJB1 Antibody) at Dilution 1:100 (under 10x lens)

SwissProt ID

P25685 (Human), Q3MI00 (Bovin), Q9QYJ3 (Mouse)

Gene ID

3337 (DNAJB1), 3337889 (Q5L6N0)

Ncbi ID

NP_006136, 5874, NP_006136.1, 9606

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Fi (Fish), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Dot blot (Dot), ELISA (detection) (E(detection))

Background of DNAJB1 / HSP40 antibody

Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

General readings

1. Melville, M. W. et al. (1997) PNAS USA, 94: 97-102.
2. Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86.
3. Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647.
4. Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261:1782-1785.
5. Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.
6. Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197:235-240. 
7. 1. Xiang-Yang Zhong, Jian-Hua Ding,Joseph A. Adams,Gourisankar Ghosh and Xiang-Dong Fu. Regulation of SR protein hosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones. Genes Dev. 2009 23: 482-495. doi: 10.1101/gad.1752109

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
13174-1-AP

DNAJB1 / HSP40 antibody

Immunofluorescent analysis of Hela cells, using 13174-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Rabbit Aff - Purified Hu, Ms E, P, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
+3 additional images
 
AM05642PU-N

DNAJB1 / HSP40 antibody

Western blot analysis on Jurkat cells: Cell lysates of Jurkat (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse k1C7 Purified Hu E, WB
0.1 mg / €340.00
  IgG2b   Acris Antibodies GmbH
+1 additional image
 
AM05642PU-S

DNAJB1 / HSP40 antibody

Western blot analysis on Jurkat cells: Cell lysates of Jurkat (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse k1C7 Purified Hu E, WB
50 µl / €220.00
  IgG2b   Acris Antibodies GmbH
+1 additional image
 
AM12022PU-N

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Mouse 3B9.E6 Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, WB
0.1 mg / €350.00
  IgG1   Acris Antibodies GmbH
 
AM12022PU-S

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Mouse 3B9.E6 Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, WB
25 µg / €250.00
  IgG1   Acris Antibodies GmbH
 
AP03016SU-N

DNAJB1 / HSP40 antibody

Western blot analysis of Hsp40 in HeLa cell lysates using a 1/1000 dilution of DNAJB1 / HSP40 Antibody Cat.-No AP03016SU Rabbit Serum IP, WB
0.1 ml / €370.00
    Acris Antibodies GmbH
 
AP03016SU-S

DNAJB1 / HSP40 antibody

Western blot analysis of Hsp40 in HeLa cell lysates using a 1/1000 dilution of DNAJB1 / HSP40 Antibody Cat.-No AP03016SU Rabbit Serum IP, WB
25 µl / €250.00
    Acris Antibodies GmbH
 
AP06173PU-N

DNAJB1 / HSP40 antibody

Immunohistochemistry analysis of DNAJB1 / HSP40 Antibody Cat.-No AP06173PU-N in paraffin-embedded lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
+1 additional image
 
AP11332PU-N

DNAJB1 / HSP40 antibody

Western blot analysis of anti-HSP40 Antibody (Cat.#AP11332PU-N) in Hela cell line lysates (35ug/lane). HSP40 (arrow) was detected using the purified Pab. Rabbit Purified Hu E, P, WB
0.1 mg / €330.00
  Ig   Acris Antibodies GmbH
+1 additional image
 
AP22890PU-N

DNAJB1 / HSP40 antibody

IHC Image for AP22890PU-N Spleen, neutrophils, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Bov, Can, Chk, Fi, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen IP, P, WB
50 µg / €380.00
    Acris Antibodies GmbH
+2 additional images
 
AP23271PU-N

DNAJB1 / HSP40 (C-term) antibody

Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining NGFbeta in cytoplasm DAB chromogenic reaction Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €340.00
  IgG   Acris Antibodies GmbH
+1 additional image
 
NB100-91818

DNAJB1 / HSP40 antibody

Western Blot: HSP40/HDJ-1 Antibody [NB100-91818] - Western blot (WB) analysis of HSP40/HDJ-1 (P303) antibody in extracts from COLO205 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  IgG   Novus Biologicals Inc.
+1 additional image
 
NBP1-04302

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Mouse k1C7 Purified Hu E, WB
0.1 ml / €340.00
  IgG2b   Novus Biologicals Inc.
 
NBP1-22416

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Mouse 2A7.H6 Purified Ye E, IP, WB
0.1 mg / €390.00
  IgG1   Novus Biologicals Inc.
 
NBP1-56645

DNAJB1 / HSP40 antibody

Western Blot: Hsp40 Antibody [NBP1-56645] - This Anti-DNAJB1 antibody was used in Western Blot of 721_B tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €390.00
    Novus Biologicals Inc.
 
NBP1-67599-0.1

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €340.00
  IgG   Novus Biologicals Inc.
 
NBP1-67599-50

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €290.00
  IgG   Novus Biologicals Inc.
 
GTX77729

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Rabbit Hu WB
0.1 mg / €330.00
  IgG   GeneTex Inc.
 
GTX102049

DNAJB1 / HSP40 antibody

Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE GTX102049 diluted at 1:1000 Rabbit N1C1 Purified Hu WB
0.1 ml / €300.00
  IgG   GeneTex Inc.
 
GTX102089

DNAJB1 / HSP40 antibody

DNAJB1 / HSP40 Aff - Purified Hu Dot
0.1 ml / €200.00
    GeneTex Inc.
 

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