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p53 (TP53) antibody

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Principal name

p53 (TP53) antibody

Alternative names for p53 (TP53) antibody

Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13, FocusOn129

Selected pictures

p53 (TP53) antibody - AM00166PU-N
AM00166PU-N - Western blot analysis of p53 in Cos cell lysate.
p53 (TP53) antibody - AP00224PU-N p53 (TP53)
AP00224PU-N p53 (TP53) - Western blot analysis of extract from UV treated (Lane 2) and untreated(Lane 1) HeLa cells.

SwissProt ID

P02340 (Mouse), P04637 (Human), P07193 (Xenla), P10360 (Chick), P10361 (Rat), P41685 (Felca), P51664 (Sheep), P67939 (Bovin), P79892 (Horse), Q29537 (Canfa), Q95330 (Rabit), Q9TUB2 (Pig), Q9WUR6 (Cavpo)

Gene ID

7157, 22059 (Trp53), 397926 (tp53), 24842 (Tp53), 4747 (NEFL)

Ncbi ID

NP_000537.3, 9606, NP_000537, NP_035770, NP_001120705.1, NP_001120705, 10090, NP_001081567, NP_112251.2, NP_006149

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Bov (Bovine), Eq (Equine), Fe (Feline), Sh (Sheep), Can (Canine), Por (Porcine), Rb (Rabbit), Av (Birds), Xen (Xenopus), Mky (Monkey), Hst (Hamster), Frog

Available hosts

Rabbit, Mouse, Sheep, Rat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Radioimmunoassay (R), Flow Cytometry (F), ELISA (detection) (E(detection)), Dot blot (Dot), ChIP assay (ChIP), Radial Immunodiffusion (RID), ELISA (capture) (E(capture))

Background of p53 (TP53) antibody

The 393 amino acid tumor suppressor phosphoprotein p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. The level of p53 protein is low in normal cells but increases following DNA damage or cellular stress. Mutations in the p53 gene are found in more than 50% of human cancers. p53 acts to arrest the cell cycle or induce apoptosis by regulating the transcription of specific genes. p53 can also initiate apoptosis through a direct signaling pathway. The p53 tumor suppressor gene encodes a transcription factor that contributes to several cellular activities that include apoptosis, transient growth arrest, and sustained growth arrest or senescence. Mutations within the p53 gene are found in about half of all human cancers. In cells that are functioning normally the MDM2 protein binds to p53 and maintains p53 at low levels by increasing its susceptibility to proteolysis by the 26S proteosome. A cell that undergoes stress loses the ability of MDM2 to bind to p53 and as a result p53 levels increase which then leads to cell cycle arrest or apoptosis. p53 induced cell cycle arrest or apoptosis can be achieved through transcriptional regulation of several genes including the cell cycle inhibitor p21, DNA repair gene GADD45, and the apoptotic inducer Bax. Besides MDM2 inactivation, p53 can also be functionally inactivated by mutation or binding to DNA tumor virus encoded proteins, such as SV40 large T antigen, Adenovirus E1B and papilloma virus E6 proteins.

General readings

1. Bartek J, Bartkova J, Vojtesek B, Staskova Z, Rejthar A, Kovarik J, Lane DP: Patterns of expression of the p53 tumour suppressor in human breast tissues and tumours in situ and in vitro. Int J Cancer 46, 839-844 (1990)
2. Bartek J, Bartkova J, Lukas J, Staskova Z, Vojtesek B, Lane DP: Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies. J Pathol 169, 27-34 (1993)
3. Fisher CJ, Gillett CE, Vojtesek B, Barnes DM, Millis RR: Problems with p53 immunohistochemcal staining: the effect of fixation and variation in the methods of evaluation. Br J Cancer 60, 26-31 (1994)
4. Petersen G, Song D, Hügle-Dörr B, Oldenburg I, Bautz EKF: Mapping of linear epitopes recognized by monoclonal antibodies with genefragment phage display libraries. Mol Gen Genet 249, 425-431 (1995)
5. Stephen CW, Helminen P, Lane DP: Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibodypeptide interactions. J Mol Biol 248, 58-78 (1995)
6. Andl T, Kahn T, Pfuhl A, Nicola T, Erber R, Conradt C, Klein W, Helbig M, Dietz A, Weidauer H, Bosch FX: Etiological involvement of oncogenic human papillomavirus in tonsillar squamous cell carcinomas lacking retinoblastoma cell cycle control. Cancer Res. 58, 5-13 (1998)
7. Homann N, Nees M, Conradt C, Dietz A, Weidauer H, Maier H, Bosch FX: Overexpression of p53 in tumor-distant epithelia of head and neck cancer patients is associated with an increased incidence of second primary carcinoma. Clin Cancer Res 7, 290-296 (2001)
8. Ohtake S, Cheng J, Ida H, Suzuki M, Ohshiro K, Zhang W, Saku T: Precancerous foci in pleomorphic adenoma of the salivary gland: recognition of focal carcinoma and atypical tumor cells by p53 immunohistochemistry. J Oral Pathol Med 31, 590-597 (2002)
9. Zarka TA, Han AC, Edelson MI, Rosenblum NG: Expression of cadherins, p53, and BCL2 in small cell carcinomas of the cervix: Potential tumor suppressor role for N-cadherin. Int J Gynecol Cancer 13, 240-243 (2003)

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439 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
DB 002-0.1

p53 (TP53) (C-term) antibody

Anti-p53 (DB 002). Western blot of p53 in mouse brain crude lysate (50μg of protein loaded). Rabbit S11-K Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 ml / €550.00
  IgG   DB Biotech, spol. s r.o.
 
DB 002-0.05

p53 (TP53) (C-term) antibody

Anti-p53 (DB 002). Western blot of p53 in mouse brain crude lysate (50μg of protein loaded). Rabbit S11-K Aff - Purified Hu, Ms, Rt E, IP, WB
50 µl / €310.00
  IgG   DB Biotech, spol. s r.o.
 
DB 026-0.1

p53 (TP53) (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human colon carcinoma tissue (4 µm) stained with p53 antibody (DB 026) shows positive nuclear immunostaining of tumor cells. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia. Rabbit M26-A Aff - Purified P
0.1 ml / €270.00
  IgG   DB Biotech, spol. s r.o.
 
DB 026-0.2

p53 (TP53) (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human colon carcinoma tissue (4 µm) stained with p53 antibody (DB 026) shows positive nuclear immunostaining of tumor cells. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia. Rabbit M26-A Aff - Purified P
0.2 ml / €410.00
  IgG   DB Biotech, spol. s r.o.
 
DB 026-0.5

p53 (TP53) (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human colon carcinoma tissue (4 µm) stained with p53 antibody (DB 026) shows positive nuclear immunostaining of tumor cells. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia. Rabbit M26-A Aff - Purified P
0.5 ml / €460.00
  IgG   DB Biotech, spol. s r.o.
 
DB 026-1

p53 (TP53) (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human colon carcinoma tissue (4 µm) stained with p53 antibody (DB 026) shows positive nuclear immunostaining of tumor cells. Kindly performed and provided by Katarína Poliakova, MD and Lubomír Straka, MD, Ph. D. from Clinical Pathology. Presov, Ltd., Presov, Slovakia. Rabbit M26-A Aff - Purified P
1 ml / €660.00
  IgG   DB Biotech, spol. s r.o.
 
DB 1012

p53 (TP53) antibody

p53 (TP53) Rabbit H21-E FITC Hu F
100 Tests / €510.00
  IgG   DB Biotech, spol. s r.o.
 
10442-1-AP

p53 (TP53) antibody

Immunohistochemistry of paraffin-embedded Endometrial cancer using 10442-1-AP(P53 Antibody) at Dilution 1:100 (under 10x lens) Rabbit Aff - Purified Hu E, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
+2 additional images
 
21891-1-AP

p53 (TP53) antibody

p53 (TP53) Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
 
AM00101PU-N

p53 (TP53) pSer392 antibody

Detection of activated p53 Recombinant C-terminal fragment of p53 was incubated with Casein Kinase II in the absence (1) or presence (2) of ATP. Proteins were separarted by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Monoclonal p53 antibody (clone 9F4) (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse 9F4 Purified Hu E, WB
0.1 mg / €390.00
  IgG1   Acris Antibodies GmbH
 
AM00166PU-N

p53 (TP53) antibody

Western blot analysis of p53 in Cos cell lysate. Mouse C6A5 Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €370.00
  IgG1   Acris Antibodies GmbH
 
AM00484PU-N

p53 (TP53) (Wild type + Mutant) (46-55) antibody

p53 (TP53) Mouse Pab 1801 Purified Hu IP, P, WB
0.1 mg / €410.00
  IgG1   Acris Antibodies GmbH
 
AM03100PU-N

p53 (TP53) pSer392 antibody

Figure 1. Immunohistochemistry staining of Wild-type p53 expressed in Human Trophoblast (Paraffin-Embedded Sections): 1A: anti-p53 antibody (Total), 1B: anti-p53 antibody (Phospho Ser392). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive. Mouse FP3.2 Aff - Purified Hu P, WB
0.1 mg / €240.00
  IgG1   Acris Antibodies GmbH
 
AM05279PU-N

p53 (TP53) (16-25) antibody

Figure 1. Western blot analysis using p53 antibody (AM05279PU-N) at 10 µg/ml on HCT116 cell lysate (Lane 1), HCT116 cell lysate activated with adriamycin (Lane 2), p21-/- cell lysate (Lane 3), P21-/- cell lysate activated with ADR (Lane 4) and p53-/- activated with ADR (Lane 5). ADR activates p53 in cells. Also shown is a graphic representation of the epitope location. Mouse X77 Purified Hu, Ms, Xen E, IP, WB
0.1 mg / €300.00
  IgG1   Acris Antibodies GmbH
 
AM05327PU-N

p53 (TP53) (371-380) antibody

Figure 1. Western blot analysis using p53 antibody (AM05327PU-N) at 1 µg/ml on native H1299 cells (Lane 1) and H1299 cells transfected with human p53 (Lane 2). Mouse HR231 Purified Hu, Mky, Ms, Rt C, E, IP, WB
0.1 mg / €300.00
  IgG2b   Acris Antibodies GmbH
+1 additional image
 
AM05328PU-N

p53 (TP53) (371-380) antibody

p53 (TP53) Mouse Pab122 Purified Hst, Hu, Mky, Ms, Rt F, ICC/IF, IP
0.1 mg / €300.00
  IgG2b   Acris Antibodies GmbH
 
AM06330SU-N

p53 (TP53) antibody

Immunohistochemical analysis of paraffin-embedded human esophageal cancer (left) and lung cancer (right), showing nuclear localization using p53 antibody Cat.-No AM06330SU-N with DAB staining. Mouse 4A8 Ascites Hu P, WB
0.1 ml / €370.00
  IgG1   Acris Antibodies GmbH
+1 additional image
 
AM08294PU-N

p53 (TP53) antibody

Immunohistochemistry (Formalin-Fixed Paraffin-Embedded (FFPE)- p53 antibody: Staining p53 in paraffin embedded human Brain, cortex by Immunohistochemistry. Mouse n.a Purified Can, Hu, Ms, Rt E, IP, P, WB
50 µg / €380.00
  IgG2a   Acris Antibodies GmbH
 
AM09102PU-N

p53 (TP53) (Wild type + Mutant) (20-31) antibody

p53 (TP53) Mouse Bp53-11 Aff - Purified Hu C, E, ICC/IF, IP, P, WB
25 µg / €250.00
  IgG2a   Acris Antibodies GmbH
 
AM10034FC-N

p53 (TP53) antibody

p53 (TP53) Mouse P53221 FITC Hu C, F, ICC/IF, P
0.2 mg / €330.00
  IgG2a   Acris Antibodies GmbH
 

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