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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-galactosidase tag antibody

Western blotting using  fluorescein conjugated anti-Beta-galactosidase  tag antibody shows a band at ~117 kDa (lanes 1-3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for bluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase Cat.-No. R1064F diluted to 1/10,000. Reaction occurred for 2 hours at RT. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® imaging system. Other detection systems will yield similar results. Rabbit FITC E. coli ICC/IF, WB
0.1 mg / €460.00
  OriGene Technologies GmbH

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