Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GFP antibody

Lineage tracing of EGFP expressing migrating pectoral girdle myogenic precursors in the chicken embryos via electroporation using TOL-2-GFP plasmid system. Vibratome sections stained with anti-GFP antibody Cat.-No. TP401 show the labeled muscle fiber soft hepectoral girdle in chicken embryos at stage HH27.
Data was kindly provided by: Nargis Khalida, Rizwan Rehimi: Institute of Anatomy, Dept. of Molecular Embryology, Ruhr University Bochum, Germany Rabbit Purified A. victoria E, IP, WB
0.2 mg / €290.00
  Torrey Pines Biolabs

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of GFP protein detected with polyclonal anti-GFP antibody. Wild type GFP (0.1 μg) was used to spike 30 μg of a HeLa whole cell lysate. This antibody detects a 27 kDa band corresponding to the epitope tag GFP. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4° C with the primary antibody diluted in 5% BLOTTO to 1:1,000, followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified A. victoria C, E, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GFP antibody

GFP Goat AP A. victoria C, E, WB
1 mg / €650.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Polyclonal anti-GFP antibody at a 1/1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1/300. Panel A shows a lateral view (ventral left). Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up.
Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO. Goat Biotin A. victoria C, E, ICC/IF, WB
1 mg / €540.00
  Acris Antibodies GmbH

GFP antibody

GFP Goat FITC A. victoria C, E, ICC/IF, WB
1 mg / €540.00
  Acris Antibodies GmbH

GFP antibody

Western Blot of Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (Mouse) Monoclonal Antibody Peroxidase Conjugate (Cat.-No R1461HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. Mouse IgG2a 9F9.F9 HRP A. victoria C, E, WB
1 mg / €730.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Rabbit IgG Biotin A. victoria C, E, WB
0.1 mg / €460.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Rabbit IgG FITC A. victoria F, ICC/IF
0.1 mg / €460.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Rabbit IgG HRP A. victoria C, E, WB
0.1 mg / €460.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of GFP protein detected with polyclonal anti-GFP antibody. Lane 1 shows negative control staining of 20 µg of mouse spleen lysate. Lane 2 shows staining of mouse spleen lysate spiked with 50 ng of wt GFP. This antibody detects a 27 kDa band corresponding to the GFP epitope tag commonly used in recombinant constructs. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE followed by transfer to nitrocellulose using standard methods. After blocking with 5% BSA in PBS, the membrane was probed for 2 h at room temperature with the primary antibody diluted in 5% BSA to 2 µg/ml, followed by washes and reaction with a 1:20,000 dilution of IRDye(TM)800 Conjugated Affinity
Purified anti-Chicken IgG [H&L] [Goat] MX10. The IRDye®(R)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Chicken Ig Aff - Purified A. victoria E, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

Western blot of GFP protein detected with polyclonal anti-GFP antibody. Lane 1 shows negative control staining of 20 µg of mouse spleen lysate. Lane 2 shows staining of mouse spleen lysate spiked with 50 ng of wt GFP. This antibody detects a 27 kDa band corresponding to the GFP epitope tag commonly used in recombinant constructs. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE followed by transfer to nitrocellulose using standard methods. After blocking with 5% BSA in PBS, the membrane was probed for 2 h at room temperature with the primary antibody diluted in 5% BSA to 2 µg/ml, followed by washes and reaction with a 1:20,000 dilution of IRDye(TM)800 Conjugated Affinity
Purified anti-Chicken IgG [H&L] [Goat] MX10. The IRDye®(R)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Chicken Ig Aff - Purified A. victoria E, ICC/IF, WB
25 µl / €190.00
  Acris Antibodies GmbH

GFP (Ads. to Hu, Ms, Rt Serum Proteins) antibody

GFP Chicken Ig Aff - Purified A. victoria E, WB
0.3 mg / €570.00
  Acris Antibodies GmbH

GFP antibody

GFP Mouse IgG2a 9F9.F9 AP A. victoria C, E, WB
1 mg / €850.00
  Acris Antibodies GmbH

GFP antibody

GFP Mouse IgG2a 9F9.F9 Biotin A. victoria C, E, WB
1 mg / €730.00
  Acris Antibodies GmbH

GFP antibody

GFP Mouse IgG2a 9F9.F9 FITC A. victoria ICC/IF
1 mg / €730.00
  Acris Antibodies GmbH

GFP antibody

Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. Anti-GFP detects a 27 kDa band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye® 800 conjugated Goat-a-Mouse IgG [H&L]IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Mouse IgG2a 9F9.F9 Purified A. victoria C, E, F, WB
1 mg / €650.00
  Acris Antibodies GmbH

GFP antibody

GFP Mouse IgG2a 9F9.F9 Texas Red A. victoria E, ICC/IF, WB
1 mg / €730.00
  Acris Antibodies GmbH

17 Item(s)

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