Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-galactosidase tag antibody

Western blot of rabbit anti-Beta-galactosidase (E. coli) antibody biotin conjugated. Lane 1: Beta-galactosidase. Load: 50 ng per lane. Primary antibody: Beta-galactosidase (E. coli) antibody biotin conjugated Cat.-No. R1064B) at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer ( for fluorescent Western blotting) for 30 min at RT. Predicted/observed size: 116 kDa, 116 kDa for Beta-galactosidase. Rabbit Biotin E. coli C, E, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Western blotting using  fluorescein conjugated anti-Beta-galactosidase  tag antibody shows a band at ~117 kDa (lanes 1-3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for bluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase Cat.-No. R1064F diluted to 1/10,000. Reaction occurred for 2 hours at RT. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® imaging system. Other detection systems will yield similar results. Rabbit FITC E. coli ICC/IF, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Western blotting using Beta-galactosidase antibody Cat.-No R1064PS. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for N<em>on-Reducing Conditions</em> of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under <em>Reducing Conditions</em> are shown on the right. Blots were blocked overnight at 4°C with blocking buffer for fluorescent Western blotting (p/n MB-070). The membrane was probed with Beta-galactosidase antibody Cat.-No R1064P diluted to 1/10,000. Reaction occurred overnight at 4°C. Dylight649™ conjugated goat-anti-rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center). Fluorescence image was captured using the VersaDoc® imaging system developed by BIO-RAD. Other detection systems will yield similar results. Rabbit Purified E. coli C, E, ICC/IF, IP, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Beta-galactosidase tag (Center) antibody

Western blot of purified E. coli  beta-galactosidase sample; reduced sample, immunostained by mAb BG-02 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 BG-02 Purified E. coli ICC/IF, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Immunohistochemical detection of beta-galactosidase on paraffin embedded section of pCDNA3-LacZ/293T cells with AM26630AF-N. Mouse IgG1 5A3 Azide Free E. coli ICC/IF, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG Azide Free E. coli Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €240.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG Biotin E. coli Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €350.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG Aff - Purified E. coli Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €440.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Figure 1. Western blotting using Fluorescein conjugated anti-Beta-galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were  resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to Nitrocellulose and blocking using blocking buffer for fluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase (Cat.-No. R1064F) diluted to 1/10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System. Other detection systems will yield similar results. Rabbit Serum E. coli C, E, P, WB
2 ml / €450.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit HRP E. coli C, E, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 2E9 Purified Aspergillus, E. coli E
1 mg / €310.00
  HyTest Ltd.

11 Item(s)

per page
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