Antibodies

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AP1 complex subunit gamma-1 / AP1G1 (646-659) antibody

  Goat polyclonal anti-AP1G1 antibody  
WB using Anti-AP1G1 antibody shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 µg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 µg of each lysate. Primary antibody was used at 1:500. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG. Goat IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

AP2A2 / HIP9 (3-14) antibody

  Goat polyclonal anti-AP2A antibody  
WB using Anti-AP2A antibody shows detection of a band just below 100 kDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 µg/ml of the immunizing peptide which effect-ively blocks the specific reactivity of this antibody with AP2A. Primary antibody was used at 1:500. Goat IgG Aff - Purified Can, Chimp, Chk, Hu, Ms, Orang-Utan, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Apelin Receptor (C-term) antibody

  Apelin Receptor / APJ Rabbit Polyclonal (C-Terminus) Antibody  
Apelin Receptor Rabbit Aff - Purified Eq, Hst, Hu, Orang-Utan, Rb, Rt C, ICC/IF, P
50 µg / €400.00
  OriGene Technologies, Inc.

CAMK2A (6-23) antibody

  Rabbit polyclonal CaM Kinase II antibody  
WB using Anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). The primary antibody diluted to 1:1,500 for 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at room temperature. Rabbit IgG Aff - Purified Can, Chk, Frog, Hu, Ms, Orang-Utan, Por, Rb, Rt, Ze E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

CGRP type 1 receptor / CRLR (N-term) antibody

  CALCRL / CRLR Rabbit Polyclonal (N-Terminus) Antibody  
CGRP type 1 receptor / CRLR Rabbit Aff - Purified Gorilla, Hu, Mky, Ms, Orang-Utan, Rb, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

Collagen type IV antibody

Collagen type IV Rabbit Purified Ms, Orang-Utan E, ICC/IF, P
0.1 ml / €480.00
  Acris Antibodies GmbH

DNA polymerase kappa (817-830) antibody

  Goat polyclonal anti-POLK antibody  
WB using Anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead).  Approximately 35 µg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature. Goat IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GGA3 (400-415) antibody

  Rabbit polyclonal anti-GGA3 antibody  
WB using Anti-GGA3 antibody shows detection of a band at ~110 kDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cells over- expressing the recombinant protein (lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane 3). MW markers are shown in lane 1 (700 nm channel - red).The primary antibody was diluted to 1:600. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Orang-Utan E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GPR85 / SREB2 (2nd Extracell. Dom.) antibody

  Rabbit Polyclonal Anti-GPR85 Antibody (Extracellular Domain)  
GPR85 / SREB2 Rabbit Aff - Purified Chk, Fi, Hu, L, Ms, Orang-Utan, Rt, Tr, Xen P
50 µg / €400.00
  OriGene Technologies, Inc.

HMGN1 / HMGN2 pSer20/24 antibody

  Rabbit polyclonal HMGN phospho S20/S24 antibody  
WB using Anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. The primary antibody was diluted 1:1,000. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, Orang-Utan, Por, Rt, Xen E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

LGR4 / GPR48 antibody

  Mouse monoclonal anti-LGR4 antibody  
Anti-LGR4 monoclonal antibody was used diluted to 5 ug/ml to detect LGR4 staining at the membrane of cells in various human tissues. A. Brain cerebellum. B. Pancreas islet. Mouse IgG2b 6G8.B3.G5.C3 Purified Chimp, Hu, Macaque, Orang-Utan E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

LIMK1 (630-647) antibody

  Rabbit polyclonal LIM Kinase antibody  
WB using Anti-LIM Kinase antibody shows detection of a 73 kDa band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kDa is unknown. Rabbit IgG Aff - Purified Chimp, Hu, Ms, Orang-Utan, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MYB antibody

Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Rabbit IgG Aff - Purified Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

MYB antibody

Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Rabbit IgG Aff - Purified Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

NIPBL (344-356) antibody

  Goat polyclonal anti-IDN3 antibody  
WB using Anti-IDN3 antibody shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Primary antibody was used at 1:1,500. Second antibody was a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L]. Goat IgG Aff - Purified Bov, Can, Hu, Ms, Orang-Utan, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

RBL2 / p130 (SPA310) antibody

  Rabbit polyclonal pRb2 p130 antibody  
WB using Anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4?. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4?. ECL was used for detection. Rabbit IgG Aff - Purified Bov, Can, Chimp, Eq, Hu, Op, Orang-Utan, Rt E, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

RFI / RNF34 (1-373) antibody

  Rabbit polyclonal anti-RNF34 antibody  
WB using anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Rabbit IgG Purified Bov, Can, Hu, Ms, Orang-Utan, Rt E, WB
0.5 mg / €419.00
  OriGene Technologies, Inc.

RIC8A (C-term) antibody

  Goat Polyclonal Antibody against RIC8A  
TA302971 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Chimp, Hu, Ms, Orang-Utan, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RNF2 (189-201) antibody

R1502P RNF2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 2.5 µg/ml followed by biotinylated anti-Goat IgG  secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Goat Aff - Purified Bov, Can, Chimp, Chk, Frog, Hu, Ms, Orang-Utan, Rt, Ze E, ICC/IF, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

SMAD1 antibody

  Rabbit polyclonal anti-SMAD1 antibody  
WB using Anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37? before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at 1:500. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG. Rabbit IgG Aff - Purified Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

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