AM03095PU-N Mouse IgG1 Isotype Control

Search for all "Mouse IgG1 Isotype Control"

0.1 mg / €190.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Mouse IgG1 Isotype Control PPV-06

AM03095PU-N

Product Description for Mouse IgG1 Isotype Control

Mouse anti Mouse IgG1 Isotype Control PPV-06.
Presentation: Aff - Purified
Product is tested for Flow Cytometry.

Properties for Mouse IgG1 Isotype Control

Product Category Isotype Controls
Target Category
Quantity 0.1 mg
Presentation Aff - Purified
Applications F
Clonality Monoclonal
Clone PPV-06
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Flow Cytometry.
Control Experiments: The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.
For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
The Azide Free derivative is cited in:
  1. Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, et al. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment. Sci Rep. 2015 Mar 9;5:8919. doi: 10.1038/srep08919. PubMed PMID: 25747469. (Free PMC Article available, 5 images available)
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein-A Affinity Chromatography
Buffer System:
PBS, pH~7.4
Preservatives:
15 mM Sodium Azide
State:
Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Aff - Purified
Specificity
Specificity:
This Monoclonal antibody clone PPV-06 reacts with undefined epitope on a plant pathogen.
The Monoclonal antibody clone PPV-06 does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general non-specific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the non-specific binding of any antibody is abolished.
Negative Species: Human, Porcine, Mouse, Rat, Other species Not tested (expected negative).

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