AM12069PP-N Mouse IgG1 Isotype Control

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0.1 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Mouse IgG1 Isotype Control MOPC-21

AM12069PP-N

Product Description for Mouse IgG1 Isotype Control

Mouse anti Mouse IgG1 Isotype Control MOPC-21.
Presentation: PerCP
Product is tested for Flow Cytometry.

Properties for Mouse IgG1 Isotype Control

Product Category Isotype Controls
Quantity 0.1 mg
Presentation PerCP
Applications F
Clonality Monoclonal
Clone MOPC-21
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Concentration 0.1 mg/ml
General Readings
  1. Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, et al. DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25. PubMed PMID: 17283169.
  2. Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. doi: 10.1182/blood-2007-07-099549. Epub 2008 Mar 12. PubMed PMID: 18337560. (Free PMC Article available, 7 images available)
  3. Yates J, Rovis F, Mitchell P, Afzali B, Tsang J, Garin M, et al. The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99. Epub 2007 Jun 1. PubMed PMID: 17545278.
  4. Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35.
  5. Bryceson YT, March ME, Barber DF, Ljunggren HG, Long EO. Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J Exp Med. 2005 Oct 3;202(7):1001-12. PubMed PMID: 16203869. (Free PMC Article available, 8 images available)
  6. Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, et al. Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936. doi: 10.1371/journal.pone.0001936. PubMed PMID: 18398462. (Free PMC Article available, 6 images available)
Storage Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light. Shelf life: one year from despatch.
Format
Label:
Conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The reagent is free of unconjugated PerCP.
PerCP
Buffer System:
Phosphate buffered saline (PBS)
Preservatives:
15 mM sodium azide
State:
Liquid Ig fraction
Species Reactivity
Species reactivity (tested):
Negative on Human and Rat
Specificity
Specificity:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.

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